Analysis of TIMP expression and activity.

Analysis of TIMP expression and activity.

This chapter provides practical information on the assay of tissue inhibitor of metalloproteinase (TIMP) activity and background information enabling meaningful interpretation of the data. Protocols are given for assessing the presence of TIMPs in biological samples by immunoblotting and by virtue o...

תיאור מלא

מידע ביבליוגרפי
Main Authors:	Troeberg, L, Nagase, H
פורמט:	Journal article
שפה:	English
יצא לאור:	2007

פריטים דומים

E. coli expression of TIMP-4 and comparative kinetic studies with TIMP-1 and TIMP-2: insights into the interactions of TIMPs and matrix metalloproteinase 2 (gelatinase A).
מאת: Troeberg, L, et al.
יצא לאור: (2002)

LRP-dependent and -independent internalisation of TIMP-3
מאת: Scilabra, S, et al.
יצא לאור: (2011)

Calcium pentosan polysulfate is a multi-functional aggrecanase inhibitor that enhances interaction with endogenous TIMP-3 in cartilage
מאת: Troeberg, L, et al.
יצא לאור: (2009)

Endocytic pathways of TIMP-3 and its N-terminal inhibitory domain
מאת: Scilabra, S, et al.
יצא לאור: (2009)

Sulfated glycosaminoglycans control the extracellular trafficking and the activity of the metalloprotease inhibitor timp-3
מאת: Troeberg, L, et al.
יצא לאור: (2014)

Sulfated glycosaminoglycans control the extracellular trafficking and the activity of the metalloprotease inhibitor TIMP-3.
מאת: Troeberg, L, et al.
יצא לאור: (2014)

Calcium pentosan polysulfate increases affinity between aggrecanases (ADAMTS-4 and-5) and TIMP-3 by a conformational change
מאת: Troeberg, L, et al.
יצא לאור: (2010)

Purification of MMPs and TIMPs.
מאת: Shimokawa, K, et al.
יצא לאור: (2010)

Purification of MMPs and TIMPs.
מאת: Shimokawa, K, et al.
יצא לאור: (2001)

The C-terminal domains of ADAMTS-4 and ADAMTS-5 promote association with N-TIMP-3.
מאת: Troeberg, L, et al.
יצא לאור: (2009)

Pentosan polysulfate increases affinity between ADAMTS-5 and TIMP-3 through formation of an electrostatically driven trimolecular complex
מאת: Troeberg, L, et al.
יצא לאור: (2012)

Pentosan polysulfate increases affinity between ADAMTS-5 and TIMP-3 through formation of an electrostatically driven trimolecular complex.
מאת: Troeberg, L, et al.
יצא לאור: (2012)

Suramin inhibits osteoarthritic cartilage degradation by increasing extracellular levels of chondroprotective tissue inhibitor of metalloproteinases 3 (TIMP-3)
מאת: Chanalaris, A, et al.
יצא לאור: (2017)

Dissecting the interaction between tissue inhibitor of metalloproteinases-3 (TIMP-3) and low density lipoprotein receptor-related protein-1 (LRP-1): Development of a "TRAP" to increase levels of TIMP-3 in the tissue.
מאת: Scilabra, S, et al.
יצא לאור: (2016)

Structure and function of matrix metalloproteinases and TIMPs.
מאת: Nagase, H, et al.
יצא לאור: (2006)

Aggrecanases and TIMP-3 in osteoarthritis: structure, function and in vivo challenges
מאת: Nagase, H
יצא לאור: (2009)

Monitoring metalloproteinase activity using synthetic fluorogenic substrates.
מאת: Troeberg, L, et al.
יצא לאור: (2004)

Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TIMPs) - Physiological roles and structural basis of MMP inhibition by TIMP-1
מאת: Nagase, H, et al.
יצא לאור: (2000)

MMP-13 is constitutively produced in human chondrocytes and co-endocytosed with ADAMTS-5 and TIMP-3 by the endocytic receptor LRP1
מאת: Yamamoto, K, et al.
יצא לאור: (2016)

Measurement of matrix metalloproteinase activities in the medium of cultured synoviocytes using zymography.
מאת: Troeberg, L, et al.
יצא לאור: (2003)

Generation of Tamoxifen inducible mice to express hTIMP-3 in articular chondrocytes to block degradation of cartilage in osteoarthritis
מאת: Lo Cascio, L, et al.
יצא לאור: (2012)

The expression of tissue inhibitor of metalloproteinase 2 (TIMP-2) is required for normal development of zebrafish embryos.
מאת: Zhang, J, et al.
יצא לאור: (2003)

Designing TIMP (tissue inhibitor of metalloproteinases) variants that are selective metalloproteinase inhibitors.
מאת: Nagase, H, et al.
יצא לאור: (2003)

The tissue inhibitors of metalloproteinases (TIMPs): an ancient family with structural and functional diversity.
מאת: Brew, K, et al.
יצא לאור: (2010)

Characterisation of N-TIMP-3 mutants that selectively inhibit the ADAMTSs
מאת: Lim, N, et al.
יצא לאור: (2009)

Individual Timp deficiencies differentially impact pro-MMP-2 activation.
מאת: English, J, et al.
יצא לאור: (2006)

TIMP-3 inhibits the procollagen N-proteinase ADAMTS-2.
מאת: Wang, WM, et al.
יצא לאור: (2006)

Increased TIMP-3 expression alters the cellular secretome through dual inhibition of the metalloprotease ADAM10 and ligand-binding of the LRP-1 receptor
מאת: Scilabra, SD, et al.
יצא לאור: (2018)

Matrix metalloproteinase expression and function during fin regeneration in zebrafish: analysis of MT1-MMP, MMP2 and TIMP2.
מאת: Bai, S, et al.
יצא לאור: (2005)

Proteases involved in cartilage matrix degradation in osteoarthritis.
מאת: Troeberg, L, et al.
יצא לאור: (2012)

Zymography of metalloproteinases.
מאת: Troeberg, L, et al.
יצא לאור: (2004)

Steps involved in activation of the complex of pro-matrix metalloproteinase 2 (progelatinase A) and tissue inhibitor of metalloproteinases (TIMP)-2 by 4-aminophenylmercuric acetate.
מאת: Itoh, Y, et al.
יצא לאור: (1995)

MMP-2 and MMP-9 Activities and TIMP-1 and TIMP-2 Expression in the Prostatic Tissue of Two Ethanol-Preferring Rat Models
מאת: Beatriz Aparecida Fioruci-Fontanelli, et al.
יצא לאור: (2015-01-01)

Identification of the role of TG2 on the expression of TGF-β, TIMP-1 and TIMP-2 in aged skin
מאת: Ergülen Elvan, et al.
יצא לאור: (2024-02-01)

Comprehensive analysis of expression, prognosis and immune infiltration for TIMPs in glioblastoma
מאת: Jinkun Han, et al.
יצא לאור: (2021-11-01)

Low density lipoprotein receptor-related protein mediates endocytic clearance of pro-MMP-2.TIMP-2 complex through a thrombospondin-independent mechanism.
מאת: Emonard, H, et al.
יצא לאור: (2004)

THE TYPE III 8-10 REPEATS OF FIBRONECTIN INDUCE AGGRECANASE ACTIVITY VIA ALPHA5BETA1 INTEGRIN AND THE TYPE III 13-14 REPEATS ACT VIA TLR 4 RECEPTORS
מאת: Sofat, N, et al.
יצא לאור: (2009)

The type III 8-10 repeats of fibronectin induce aggrecanase activity via alpha(5)beta(1) integrin and type III 13-14 repeats act via TLR 4 receptors
מאת: Sofat, N, et al.
יצא לאור: (2009)

Selective inhibition of ADAM12 catalytic activity through engineering of tissue inhibitor of metalloproteinase 2 (TIMP-2).
מאת: Kveiborg, M, et al.
יצא לאור: (2010)

Differential inhibition of membrane type 3 (MT3)-matrix metalloproteinase (MMP) and MT1-MMP by tissue inhibitor of metalloproteinase (TIMP)-2 and TIMP-3 rgulates pro-MMP-2 activation.
מאת: Zhao, H, et al.
יצא לאור: (2004)