Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.
UCHL1 is a murine monoclonal antibody that recognises a 180-185 kD determinant on CD4 (72%) and CD8 (36%) positive T cells. This antibody is effective in formalin fixed and paraffin embedded tissues, using the immunoperoxidase method. One hundred and forty three cases of malignant lymphoma were exam...
| Päätekijät: | , , , , |
|---|---|
| Aineistotyyppi: | Journal article |
| Kieli: | English |
| Julkaistu: |
1986
|
| _version_ | 1826289100562366464 |
|---|---|
| author | Norton, A Ramsay, A Smith, S Beverley, P Isaacson, P |
| author_facet | Norton, A Ramsay, A Smith, S Beverley, P Isaacson, P |
| author_sort | Norton, A |
| collection | OXFORD |
| description | UCHL1 is a murine monoclonal antibody that recognises a 180-185 kD determinant on CD4 (72%) and CD8 (36%) positive T cells. This antibody is effective in formalin fixed and paraffin embedded tissues, using the immunoperoxidase method. One hundred and forty three cases of malignant lymphoma were examined. Neoplastic cells in 100% of cases of Mycosis fungoides (n = 10), 83% of cases of peripheral T cell lymphoma (n = 25), and 78% of cases of (T-ALL) T acute lymphoblastic lymphoma (n = 9) were stained by this antibody. In addition, staining was seen in 100% of cases of malignant histiocytosis of the intestine (n = 13), a condition now thought to be a T cell lymphoma. Two cases of true histiocytic lymphoma were also positive. This antibody stained neither the neoplastic cells in a wide range of B cell lymphomas (n = 62) nor Reed-Sternberg cells in 16 cases of Hodgkin's disease. UCHL1 also stained neoplastic cells in four cases of granulocytic sarcoma. A panel of normal tissues was similarly studied. Staining was seen in normal T cells and mucosal intraepithelial lymphocytes, macrophages, mature myeloid cells, and endometrial stromal granulocytes. UCHL1 is a monoclonal antibody that identifies T cells in formalin fixed paraffin embedded tissues, and should prove useful for diagnosing T cell lymphomas, especially when only formalin fixed tissue is available for diagnosis. |
| first_indexed | 2024-03-07T02:23:45Z |
| format | Journal article |
| id | oxford-uuid:a4e08b08-f6ac-4caa-b79a-8104bc8dbf18 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:23:45Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:a4e08b08-f6ac-4caa-b79a-8104bc8dbf182022-03-27T02:36:43ZMonoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a4e08b08-f6ac-4caa-b79a-8104bc8dbf18EnglishSymplectic Elements at Oxford1986Norton, ARamsay, ASmith, SBeverley, PIsaacson, PUCHL1 is a murine monoclonal antibody that recognises a 180-185 kD determinant on CD4 (72%) and CD8 (36%) positive T cells. This antibody is effective in formalin fixed and paraffin embedded tissues, using the immunoperoxidase method. One hundred and forty three cases of malignant lymphoma were examined. Neoplastic cells in 100% of cases of Mycosis fungoides (n = 10), 83% of cases of peripheral T cell lymphoma (n = 25), and 78% of cases of (T-ALL) T acute lymphoblastic lymphoma (n = 9) were stained by this antibody. In addition, staining was seen in 100% of cases of malignant histiocytosis of the intestine (n = 13), a condition now thought to be a T cell lymphoma. Two cases of true histiocytic lymphoma were also positive. This antibody stained neither the neoplastic cells in a wide range of B cell lymphomas (n = 62) nor Reed-Sternberg cells in 16 cases of Hodgkin's disease. UCHL1 also stained neoplastic cells in four cases of granulocytic sarcoma. A panel of normal tissues was similarly studied. Staining was seen in normal T cells and mucosal intraepithelial lymphocytes, macrophages, mature myeloid cells, and endometrial stromal granulocytes. UCHL1 is a monoclonal antibody that identifies T cells in formalin fixed paraffin embedded tissues, and should prove useful for diagnosing T cell lymphomas, especially when only formalin fixed tissue is available for diagnosis. |
| spellingShingle | Norton, A Ramsay, A Smith, S Beverley, P Isaacson, P Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title | Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title_full | Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title_fullStr | Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title_full_unstemmed | Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title_short | Monoclonal antibody (UCHL1) that recognises normal and neoplastic T cells in routinely fixed tissues. |
| title_sort | monoclonal antibody uchl1 that recognises normal and neoplastic t cells in routinely fixed tissues |
| work_keys_str_mv | AT nortona monoclonalantibodyuchl1thatrecognisesnormalandneoplastictcellsinroutinelyfixedtissues AT ramsaya monoclonalantibodyuchl1thatrecognisesnormalandneoplastictcellsinroutinelyfixedtissues AT smiths monoclonalantibodyuchl1thatrecognisesnormalandneoplastictcellsinroutinelyfixedtissues AT beverleyp monoclonalantibodyuchl1thatrecognisesnormalandneoplastictcellsinroutinelyfixedtissues AT isaacsonp monoclonalantibodyuchl1thatrecognisesnormalandneoplastictcellsinroutinelyfixedtissues |