cAMP: From long-range second messenger to nanodomain signalling
How cAMP generates hormone-specific effects has been debated for many decades. Fluorescence resonance energy transfer (FRET)-based sensors for cAMP allow real-time imaging of the second messenger in intact cells with high spatiotemporal resolution. This technology has made it possible to directly de...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Elsevier
2017
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| _version_ | 1826289110683222016 |
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| author | Musheshe, N Schmidt, M Zaccolo, M |
| author_facet | Musheshe, N Schmidt, M Zaccolo, M |
| author_sort | Musheshe, N |
| collection | OXFORD |
| description | How cAMP generates hormone-specific effects has been debated for many decades. Fluorescence resonance energy transfer (FRET)-based sensors for cAMP allow real-time imaging of the second messenger in intact cells with high spatiotemporal resolution. This technology has made it possible to directly demonstrate that cAMP signals are compartmentalised. The details of such signal compartmentalisation are still being uncovered, and recent findings reveal a previously unsuspected submicroscopic heterogeneity of intracellular cAMP. A model is emerging where specificity depends on compartmentalisation and where the physiologically relevant signals are those that occur within confined nanodomains, rather than bulk changes in cytosolic cAMP. These findings subvert the classical notion of cAMP signalling and provide a new framework for the development of targeted therapeutic approaches. |
| first_indexed | 2024-03-07T02:23:54Z |
| format | Journal article |
| id | oxford-uuid:a4ee80f8-14b1-4701-aed0-cc5127b5b5b5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:23:54Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:a4ee80f8-14b1-4701-aed0-cc5127b5b5b52022-03-27T02:37:02ZcAMP: From long-range second messenger to nanodomain signallingJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a4ee80f8-14b1-4701-aed0-cc5127b5b5b5EnglishSymplectic Elements at OxfordElsevier2017Musheshe, NSchmidt, MZaccolo, MHow cAMP generates hormone-specific effects has been debated for many decades. Fluorescence resonance energy transfer (FRET)-based sensors for cAMP allow real-time imaging of the second messenger in intact cells with high spatiotemporal resolution. This technology has made it possible to directly demonstrate that cAMP signals are compartmentalised. The details of such signal compartmentalisation are still being uncovered, and recent findings reveal a previously unsuspected submicroscopic heterogeneity of intracellular cAMP. A model is emerging where specificity depends on compartmentalisation and where the physiologically relevant signals are those that occur within confined nanodomains, rather than bulk changes in cytosolic cAMP. These findings subvert the classical notion of cAMP signalling and provide a new framework for the development of targeted therapeutic approaches. |
| spellingShingle | Musheshe, N Schmidt, M Zaccolo, M cAMP: From long-range second messenger to nanodomain signalling |
| title | cAMP: From long-range second messenger to nanodomain signalling |
| title_full | cAMP: From long-range second messenger to nanodomain signalling |
| title_fullStr | cAMP: From long-range second messenger to nanodomain signalling |
| title_full_unstemmed | cAMP: From long-range second messenger to nanodomain signalling |
| title_short | cAMP: From long-range second messenger to nanodomain signalling |
| title_sort | camp from long range second messenger to nanodomain signalling |
| work_keys_str_mv | AT musheshen campfromlongrangesecondmessengertonanodomainsignalling AT schmidtm campfromlongrangesecondmessengertonanodomainsignalling AT zaccolom campfromlongrangesecondmessengertonanodomainsignalling |