2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA.

Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2&#...

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Detalhes bibliográficos
Main Authors: Haugland, M, El-Sagheer, A, Porter, R, Peña, J, Brown, T, Anderson, E, Lovett, J
Formato: Journal article
Idioma:English
Publicado em: American Chemical Society 2016
Descrição
Resumo:Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information.