2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA.
Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2&#...
| Автори: | , , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
American Chemical Society
2016
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| _version_ | 1826289118992138240 |
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| author | Haugland, M El-Sagheer, A Porter, R Peña, J Brown, T Anderson, E Lovett, J |
| author_facet | Haugland, M El-Sagheer, A Porter, R Peña, J Brown, T Anderson, E Lovett, J |
| author_sort | Haugland, M |
| collection | OXFORD |
| description | Electron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information. |
| first_indexed | 2024-03-07T02:24:02Z |
| format | Journal article |
| id | oxford-uuid:a4f848a7-01c5-45d6-b0a1-7b760de5e0d5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:24:02Z |
| publishDate | 2016 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:a4f848a7-01c5-45d6-b0a1-7b760de5e0d52022-03-27T02:37:17Z2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a4f848a7-01c5-45d6-b0a1-7b760de5e0d5EnglishSymplectic Elements at OxfordAmerican Chemical Society2016Haugland, MEl-Sagheer, APorter, RPeña, JBrown, TAnderson, ELovett, JElectron paramagnetic resonance (EPR) spectroscopy is a powerful method to elucidate molecular structure through the measurement of distances between conformationally well-defined spin labels. Here we report a sequence-flexible approach to the synthesis of double spin-labeled DNA duplexes, where 2'-alkynylnucleosides are incorporated at terminal and internal positions on complementary strands. Post-DNA synthesis copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions with a variety of spin labels enable the use of double electron-electron resonance experiments to measure a number of distances on the duplex, affording a high level of detailed structural information. |
| spellingShingle | Haugland, M El-Sagheer, A Porter, R Peña, J Brown, T Anderson, E Lovett, J 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title | 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title_full | 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title_fullStr | 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title_full_unstemmed | 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title_short | 2'-alkynylnucleotides: A sequence- and spin label-flexible strategy for EPR spectroscopy in DNA. |
| title_sort | 2 alkynylnucleotides a sequence and spin label flexible strategy for epr spectroscopy in dna |
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