MLST revisited: the gene-by-gene approach to bacterial genomics.
Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2013
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| _version_ | 1797086634027515904 |
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| author | Maiden, M Jansen van Rensburg, M Bray, J Earle, S Ford, SA Jolley, K McCarthy, N |
| author_facet | Maiden, M Jansen van Rensburg, M Bray, J Earle, S Ford, SA Jolley, K McCarthy, N |
| author_sort | Maiden, M |
| collection | OXFORD |
| description | Multilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data. |
| first_indexed | 2024-03-07T02:24:42Z |
| format | Journal article |
| id | oxford-uuid:a530eeb3-ea1c-4bc1-bc8b-617eda3f9912 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:24:42Z |
| publishDate | 2013 |
| record_format | dspace |
| spelling | oxford-uuid:a530eeb3-ea1c-4bc1-bc8b-617eda3f99122022-03-27T02:38:43ZMLST revisited: the gene-by-gene approach to bacterial genomics.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a530eeb3-ea1c-4bc1-bc8b-617eda3f9912EnglishSymplectic Elements at Oxford2013Maiden, MJansen van Rensburg, MBray, JEarle, SFord, SAJolley, KMcCarthy, NMultilocus sequence typing (MLST) was proposed in 1998 as a portable sequence-based method for identifying clonal relationships among bacteria. Today, in the whole-genome era of microbiology, the need for systematic, standardized descriptions of bacterial genotypic variation remains a priority. Here, to meet this need, we draw on the successes of MLST and 16S rRNA gene sequencing to propose a hierarchical gene-by-gene approach that reflects functional and evolutionary relationships and catalogues bacteria 'from domain to strain'. Our gene-based typing approach using online platforms such as the Bacterial Isolate Genome Sequence Database (BIGSdb) allows the scalable organization and analysis of whole-genome sequence data. |
| spellingShingle | Maiden, M Jansen van Rensburg, M Bray, J Earle, S Ford, SA Jolley, K McCarthy, N MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title | MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title_full | MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title_fullStr | MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title_full_unstemmed | MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title_short | MLST revisited: the gene-by-gene approach to bacterial genomics. |
| title_sort | mlst revisited the gene by gene approach to bacterial genomics |
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