Global gene expression responses of fission yeast to ionizing radiation.
A coordinated transcriptional response to DNA-damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) by using DNA microarrays. We identified approximately 200 genes whos...
| Hlavní autoři: | , , , , |
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| Médium: | Journal article |
| Jazyk: | English |
| Vydáno: |
2004
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| _version_ | 1826289195437522944 |
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| author | Watson, A Mata, J Bähler, J Carr, A Humphrey, T |
| author_facet | Watson, A Mata, J Bähler, J Carr, A Humphrey, T |
| author_sort | Watson, A |
| collection | OXFORD |
| description | A coordinated transcriptional response to DNA-damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) by using DNA microarrays. We identified approximately 200 genes whose transcript levels were significantly altered at least twofold in response to 500 Gy of gamma IR in a temporally defined manner. The majority of induced genes were core environmental stress response genes, whereas the remaining genes define a transcriptional response to DNA damage in fission yeast. Surprisingly, few DNA repair and checkpoint genes were transcriptionally modulated in response to IR. We define a role for the stress-activated mitogen-activated protein kinase Sty1/Spc1 and the DNA damage checkpoint kinase Rad3 in regulating core environmental stress response genes and IR-specific response genes, both independently and in concert. These findings suggest a complex network of regulatory pathways coordinate gene expression responses to IR in eukaryotes. |
| first_indexed | 2024-03-07T02:25:12Z |
| format | Journal article |
| id | oxford-uuid:a55b07b5-dd1e-43f5-b8e1-9e1a7e7b782a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:25:12Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:a55b07b5-dd1e-43f5-b8e1-9e1a7e7b782a2022-03-27T02:39:53ZGlobal gene expression responses of fission yeast to ionizing radiation.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a55b07b5-dd1e-43f5-b8e1-9e1a7e7b782aEnglishSymplectic Elements at Oxford2004Watson, AMata, JBähler, JCarr, AHumphrey, TA coordinated transcriptional response to DNA-damaging agents is required to maintain genome stability. We have examined the global gene expression responses of the fission yeast Schizosaccharomyces pombe to ionizing radiation (IR) by using DNA microarrays. We identified approximately 200 genes whose transcript levels were significantly altered at least twofold in response to 500 Gy of gamma IR in a temporally defined manner. The majority of induced genes were core environmental stress response genes, whereas the remaining genes define a transcriptional response to DNA damage in fission yeast. Surprisingly, few DNA repair and checkpoint genes were transcriptionally modulated in response to IR. We define a role for the stress-activated mitogen-activated protein kinase Sty1/Spc1 and the DNA damage checkpoint kinase Rad3 in regulating core environmental stress response genes and IR-specific response genes, both independently and in concert. These findings suggest a complex network of regulatory pathways coordinate gene expression responses to IR in eukaryotes. |
| spellingShingle | Watson, A Mata, J Bähler, J Carr, A Humphrey, T Global gene expression responses of fission yeast to ionizing radiation. |
| title | Global gene expression responses of fission yeast to ionizing radiation. |
| title_full | Global gene expression responses of fission yeast to ionizing radiation. |
| title_fullStr | Global gene expression responses of fission yeast to ionizing radiation. |
| title_full_unstemmed | Global gene expression responses of fission yeast to ionizing radiation. |
| title_short | Global gene expression responses of fission yeast to ionizing radiation. |
| title_sort | global gene expression responses of fission yeast to ionizing radiation |
| work_keys_str_mv | AT watsona globalgeneexpressionresponsesoffissionyeasttoionizingradiation AT mataj globalgeneexpressionresponsesoffissionyeasttoionizingradiation AT bahlerj globalgeneexpressionresponsesoffissionyeasttoionizingradiation AT carra globalgeneexpressionresponsesoffissionyeasttoionizingradiation AT humphreyt globalgeneexpressionresponsesoffissionyeasttoionizingradiation |