Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex.
Protein homoeostasis is a fundamental requirement for all living cells in order to survive in a dynamic surrounding. Proper levels of AIRAPL (arsenite-inducible RNA-associated protein-like protein) (ZFAND2B) are required in order to maintain cellular folding capacity in metazoans, and functional imp...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2014
|
| _version_ | 1797086738300010496 |
|---|---|
| author | Glinka, T Alter, J Braunstein, I Tzach, L Wei Sheng, C Geifman, S Edelmann, M Kessler, B Stanhill, A |
| author_facet | Glinka, T Alter, J Braunstein, I Tzach, L Wei Sheng, C Geifman, S Edelmann, M Kessler, B Stanhill, A |
| author_sort | Glinka, T |
| collection | OXFORD |
| description | Protein homoeostasis is a fundamental requirement for all living cells in order to survive in a dynamic surrounding. Proper levels of AIRAPL (arsenite-inducible RNA-associated protein-like protein) (ZFAND2B) are required in order to maintain cellular folding capacity in metazoans, and functional impairment of AIRAPL results in acceleration of aging and protein aggregation. However, the cellular roles of AIRAPL in this process are not known. In the present paper, we report that AIRAPL binds and forms a complex with p97 [VCP (valosin-containing protein)/Cdc48], Ubxd8 (ubiquitin regulatory X domain 8), Npl4-Ufd1, Derlin-1 and Bag6 on the ER (endoplasmic reticulum) membrane. In spite of the fact that AIRAPL complex partners are involved in the ERAD (ER-associated degradation) process, AIRAPL knockdown does not show any impairment in ERAD substrate degradation. However, translocation into the ER of a subset of ERAD- and non-ERAD-secreted proteins are regulated by AIRAPL. The ability to regulate translocation by the p97-AIRAPL complex is entirely dependent on the proteins' signal peptide. Our results demonstrate a p97 complex regulating translocation into the ER in a signal-peptide-dependent manner. |
| first_indexed | 2024-03-07T02:26:15Z |
| format | Journal article |
| id | oxford-uuid:a5b55e32-ad7b-4f9e-8ff3-96f1e0384983 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:26:15Z |
| publishDate | 2014 |
| record_format | dspace |
| spelling | oxford-uuid:a5b55e32-ad7b-4f9e-8ff3-96f1e03849832022-03-27T02:42:18ZSignal-peptide-mediated translocation is regulated by a p97-AIRAPL complex.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a5b55e32-ad7b-4f9e-8ff3-96f1e0384983EnglishSymplectic Elements at Oxford2014Glinka, TAlter, JBraunstein, ITzach, LWei Sheng, CGeifman, SEdelmann, MKessler, BStanhill, AProtein homoeostasis is a fundamental requirement for all living cells in order to survive in a dynamic surrounding. Proper levels of AIRAPL (arsenite-inducible RNA-associated protein-like protein) (ZFAND2B) are required in order to maintain cellular folding capacity in metazoans, and functional impairment of AIRAPL results in acceleration of aging and protein aggregation. However, the cellular roles of AIRAPL in this process are not known. In the present paper, we report that AIRAPL binds and forms a complex with p97 [VCP (valosin-containing protein)/Cdc48], Ubxd8 (ubiquitin regulatory X domain 8), Npl4-Ufd1, Derlin-1 and Bag6 on the ER (endoplasmic reticulum) membrane. In spite of the fact that AIRAPL complex partners are involved in the ERAD (ER-associated degradation) process, AIRAPL knockdown does not show any impairment in ERAD substrate degradation. However, translocation into the ER of a subset of ERAD- and non-ERAD-secreted proteins are regulated by AIRAPL. The ability to regulate translocation by the p97-AIRAPL complex is entirely dependent on the proteins' signal peptide. Our results demonstrate a p97 complex regulating translocation into the ER in a signal-peptide-dependent manner. |
| spellingShingle | Glinka, T Alter, J Braunstein, I Tzach, L Wei Sheng, C Geifman, S Edelmann, M Kessler, B Stanhill, A Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title | Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title_full | Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title_fullStr | Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title_full_unstemmed | Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title_short | Signal-peptide-mediated translocation is regulated by a p97-AIRAPL complex. |
| title_sort | signal peptide mediated translocation is regulated by a p97 airapl complex |
| work_keys_str_mv | AT glinkat signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT alterj signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT braunsteini signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT tzachl signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT weishengc signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT geifmans signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT edelmannm signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT kesslerb signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex AT stanhilla signalpeptidemediatedtranslocationisregulatedbyap97airaplcomplex |