Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases
Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for the treatment of inflammatory bowerl disease, releasing the active component 5-aminosalycilic acid. The bacterium <em>P. aeruginosa,/...
| Main Authors: | , , , , |
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| Other Authors: | |
| Format: | Journal article |
| Language: | English |
| Published: |
Springer
2010
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| Subjects: |
| _version_ | 1797086779534213120 |
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| author | Ryan, A Wang, C Laurieri, N Westwood, I Sim, E |
| author2 | Higher Education Press |
| author_facet | Higher Education Press Ryan, A Wang, C Laurieri, N Westwood, I Sim, E |
| author_sort | Ryan, A |
| collection | OXFORD |
| description | Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for the treatment of inflammatory bowerl disease, releasing the active component 5-aminosalycilic acid. The bacterium <em>P. aeruginosa,/em> has three azoreductase genes, paAzoR1, paAzoR2 and paAzoR3, which as recombinant enzymes have been shown to have different substrate specificities. The mechanisms of azoreduction relies upon tautomerisation of the substrate to the hydrazone form. We report here the characterization of the <em>P. aeruginosa</em> azoreductase enzymes, including determining their thermostability, cofactor preference and kinetic constants against a range of their favoured substrates. The expression levels of these enzymes during growth of <em>P. aeruginosa</em> are altered by the presence of azo substrates. It is shown that enzymes that were originally described as azoreductases, are likely to act as NADH quinone oxidoreductases. The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution.</em> |
| first_indexed | 2024-03-07T02:26:52Z |
| format | Journal article |
| id | oxford-uuid:a5e7157c-9ff0-4c77-b0af-7d2841c3c8be |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:26:52Z |
| publishDate | 2010 |
| publisher | Springer |
| record_format | dspace |
| spelling | oxford-uuid:a5e7157c-9ff0-4c77-b0af-7d2841c3c8be2022-03-27T02:43:35ZReaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductasesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a5e7157c-9ff0-4c77-b0af-7d2841c3c8bePharmacologyEnglishOxford University Research Archive - ValetSpringer2010Ryan, AWang, CLaurieri, NWestwood, ISim, EHigher Education PressAzoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for the treatment of inflammatory bowerl disease, releasing the active component 5-aminosalycilic acid. The bacterium <em>P. aeruginosa,/em> has three azoreductase genes, paAzoR1, paAzoR2 and paAzoR3, which as recombinant enzymes have been shown to have different substrate specificities. The mechanisms of azoreduction relies upon tautomerisation of the substrate to the hydrazone form. We report here the characterization of the <em>P. aeruginosa</em> azoreductase enzymes, including determining their thermostability, cofactor preference and kinetic constants against a range of their favoured substrates. The expression levels of these enzymes during growth of <em>P. aeruginosa</em> are altered by the presence of azo substrates. It is shown that enzymes that were originally described as azoreductases, are likely to act as NADH quinone oxidoreductases. The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution.</em> |
| spellingShingle | Pharmacology Ryan, A Wang, C Laurieri, N Westwood, I Sim, E Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title_full | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title_fullStr | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title_full_unstemmed | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title_short | Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| title_sort | reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases |
| topic | Pharmacology |
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