Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum.
Artemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily availa...
| Main Authors: | , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
2004
|
| _version_ | 1826289375826149376 |
|---|---|
| author | Teja-Isavadharm, P Peggins, J Brewer, T White, N Webster, H Kyle, D |
| author_facet | Teja-Isavadharm, P Peggins, J Brewer, T White, N Webster, H Kyle, D |
| author_sort | Teja-Isavadharm, P |
| collection | OXFORD |
| description | Artemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily available and reproducible assays to monitor drug levels closely in patients. A sensitive and reproducible bioassay for the measurement of the concentrations of artemisinin derivatives in plasma and serum is described. By modifying the in vitro drug susceptibility test, it was found that antimalarial activity in plasma or serum containing an unknown concentration of drug could be equated to the known concentrations of dihydroartemisinin (DHA) required to inhibit parasite growth. Dose-response curves for a Plasmodium falciparum clone (clone W2) and DHA were used as a standard for each assay. Assays with plasma or serum spiked with DHA proved to be reproducible (coefficient of variation, |
| first_indexed | 2024-03-07T02:27:56Z |
| format | Journal article |
| id | oxford-uuid:a6434c2d-d47b-4270-838c-8bd02b5d1752 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:27:56Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:a6434c2d-d47b-4270-838c-8bd02b5d17522022-03-27T02:46:05ZPlasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a6434c2d-d47b-4270-838c-8bd02b5d1752EnglishSymplectic Elements at Oxford2004Teja-Isavadharm, PPeggins, JBrewer, TWhite, NWebster, HKyle, DArtemisinin and its derivatives, artesunate and artemether, are rapidly acting antimalarials that are used for the treatment of severe and uncomplicated multidrug-resistant falciparum malaria. To optimize treatment regimens that use this new class of antimalarials, there is a need for readily available and reproducible assays to monitor drug levels closely in patients. A sensitive and reproducible bioassay for the measurement of the concentrations of artemisinin derivatives in plasma and serum is described. By modifying the in vitro drug susceptibility test, it was found that antimalarial activity in plasma or serum containing an unknown concentration of drug could be equated to the known concentrations of dihydroartemisinin (DHA) required to inhibit parasite growth. Dose-response curves for a Plasmodium falciparum clone (clone W2) and DHA were used as a standard for each assay. Assays with plasma or serum spiked with DHA proved to be reproducible (coefficient of variation, |
| spellingShingle | Teja-Isavadharm, P Peggins, J Brewer, T White, N Webster, H Kyle, D Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title | Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title_full | Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title_fullStr | Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title_full_unstemmed | Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title_short | Plasmodium falciparum-based bioassay for measurement of artemisinin derivatives in plasma or serum. |
| title_sort | plasmodium falciparum based bioassay for measurement of artemisinin derivatives in plasma or serum |
| work_keys_str_mv | AT tejaisavadharmp plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum AT pegginsj plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum AT brewert plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum AT whiten plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum AT websterh plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum AT kyled plasmodiumfalciparumbasedbioassayformeasurementofartemisininderivativesinplasmaorserum |