Ratiometric analysis of calcium mobilization
One of the earliest events in lymphocyte activation is an increase in the concentration of cytosolic free Ca2+. Antigen recognition, chemotaxis, apoptosis and cell cycle progression are all associated with changes in cytosolic free Ca2+ levels. The kinetics, subcellular localization, and degree of C...
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| Format: | Journal article |
| Language: | English |
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2001
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| _version_ | 1797087035174944768 |
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| author | Dustin, L |
| author_facet | Dustin, L |
| author_sort | Dustin, L |
| collection | OXFORD |
| description | One of the earliest events in lymphocyte activation is an increase in the concentration of cytosolic free Ca2+. Antigen recognition, chemotaxis, apoptosis and cell cycle progression are all associated with changes in cytosolic free Ca2+ levels. The kinetics, subcellular localization, and degree of Ca2+ mobilization can be assessed with fluorescent Ca2+ indicators. Ratiometric analysis of Ca2+ mobilization reduces errors due to variations in cell size and shape, indicator loading and instrument variability that can otherwise limit the usefulness of fluorescent Ca2+ indicators. The purposes of this paper are to summarize key characteristics of some useful Ca2+ -sensitive fluorescent dyes (Indo-1, Fura-2, Fluo-3, Fluo-4 and Fura Red) and to review published methods for the optimization of Ca2+ mobilization analysis with ratiometric indicators. Analysis of Ca2+ mobilization provides a rapid, quantitative readout of lymphocyte activation. © 2000 Elsevier Science Inc. All rights reserved. |
| first_indexed | 2024-03-07T02:30:25Z |
| format | Journal article |
| id | oxford-uuid:a70b9690-31bf-4da6-94ec-9f4dc4af6651 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:30:25Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:a70b9690-31bf-4da6-94ec-9f4dc4af66512022-03-27T02:51:45ZRatiometric analysis of calcium mobilizationJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a70b9690-31bf-4da6-94ec-9f4dc4af6651EnglishSymplectic Elements at Oxford2001Dustin, LOne of the earliest events in lymphocyte activation is an increase in the concentration of cytosolic free Ca2+. Antigen recognition, chemotaxis, apoptosis and cell cycle progression are all associated with changes in cytosolic free Ca2+ levels. The kinetics, subcellular localization, and degree of Ca2+ mobilization can be assessed with fluorescent Ca2+ indicators. Ratiometric analysis of Ca2+ mobilization reduces errors due to variations in cell size and shape, indicator loading and instrument variability that can otherwise limit the usefulness of fluorescent Ca2+ indicators. The purposes of this paper are to summarize key characteristics of some useful Ca2+ -sensitive fluorescent dyes (Indo-1, Fura-2, Fluo-3, Fluo-4 and Fura Red) and to review published methods for the optimization of Ca2+ mobilization analysis with ratiometric indicators. Analysis of Ca2+ mobilization provides a rapid, quantitative readout of lymphocyte activation. © 2000 Elsevier Science Inc. All rights reserved. |
| spellingShingle | Dustin, L Ratiometric analysis of calcium mobilization |
| title | Ratiometric analysis of calcium mobilization |
| title_full | Ratiometric analysis of calcium mobilization |
| title_fullStr | Ratiometric analysis of calcium mobilization |
| title_full_unstemmed | Ratiometric analysis of calcium mobilization |
| title_short | Ratiometric analysis of calcium mobilization |
| title_sort | ratiometric analysis of calcium mobilization |
| work_keys_str_mv | AT dustinl ratiometricanalysisofcalciummobilization |