Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale
The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemic...
| Main Authors: | , , , , , , |
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| Formato: | Journal article |
| Idioma: | English |
| Publicado: |
Nature Research
2024
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| _version_ | 1826313709325123584 |
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| author | de Carvalho, LAEB Cinque, G de Carvalho, ALMB Marques, J Frogley, MD Vondracek, H Marques, MPM |
| author_facet | de Carvalho, LAEB Cinque, G de Carvalho, ALMB Marques, J Frogley, MD Vondracek, H Marques, MPM |
| author_sort | de Carvalho, LAEB |
| collection | OXFORD |
| description | The cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells. |
| first_indexed | 2024-09-25T04:19:16Z |
| format | Journal article |
| id | oxford-uuid:a72638b7-c77e-444b-87db-959f4b6eb239 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-09-25T04:19:16Z |
| publishDate | 2024 |
| publisher | Nature Research |
| record_format | dspace |
| spelling | oxford-uuid:a72638b7-c77e-444b-87db-959f4b6eb2392024-07-27T19:38:50ZSynchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scaleJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a72638b7-c77e-444b-87db-959f4b6eb239EnglishJisc Publications RouterNature Research2024de Carvalho, LAEBCinque, Gde Carvalho, ALMBMarques, JFrogley, MDVondracek, HMarques, MPMThe cellular response to cisplatin was assessed in human osteosarcoma cells, using synchrotron-based (SR) Fourier Transform InfraRed nanospectroscopy (nano-FTIR) at the MIRIAM beamline B22 of Diamond Light Source (UK). This label-free mapping method delivered simultaneous morphological and biochemical information on a subcellular level (i.e. 100 s nanometer or better). Based on specific spectral biomarkers, the main biochemical constituents affected by the drug were identified at distinct locations within the cell´s inner body. Cisplatin was shown to have a noteworthy effect on proteins, mostly within the cytoplasm. A clear drug impact on cellular lipids was also observed. Within current literature on s-SNOM, this nanospectroscopy work represents a first successful application in life sciences providing full fingerprint nano-FTIR spectra across intact human cancer cells. |
| spellingShingle | de Carvalho, LAEB Cinque, G de Carvalho, ALMB Marques, J Frogley, MD Vondracek, H Marques, MPM Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title | Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title_full | Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title_fullStr | Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title_full_unstemmed | Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title_short | Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale |
| title_sort | synchrotron nano ftir spectroscopy for probing anticancer drugs at subcellular scale |
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