Tools for decoding ubiquitin signaling in DNA repair
The maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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Frontiers Media
2021
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| _version_ | 1826310108424962048 |
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| author | Foster, B Attwood, M Gibbs-Seymour, I |
| author_facet | Foster, B Attwood, M Gibbs-Seymour, I |
| author_sort | Foster, B |
| collection | OXFORD |
| description | The maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from both exogenous and endogenous agents. Intrinsic to these mechanisms, cells have an arsenal of protein factors that can be utilised to promote repair processes in response to DNA lesions. Orchestration of the protein factors within the various cellular DNA repair pathways is performed, in part, by post-translational modifications, such as phosphorylation, ubiquitin, SUMO and other ubiquitin-like modifiers (UBLs). In this review, we firstly explore recent advances in the tools for identifying factors involved in both DNA repair and ubiquitin signaling pathways. We then expand on this by evaluating the growing repertoire of proteomic, biochemical and structural techniques available to further understand the mechanistic basis by which these complex modifications regulate DNA repair. Together, we provide a snapshot of the range of methods now available to investigate and decode how ubiquitin signaling can promote DNA repair and maintain genome stability in mammalian cells. |
| first_indexed | 2024-03-07T07:45:47Z |
| format | Journal article |
| id | oxford-uuid:a728ca7f-f82f-4a6e-9900-bfb368e13abe |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:45:47Z |
| publishDate | 2021 |
| publisher | Frontiers Media |
| record_format | dspace |
| spelling | oxford-uuid:a728ca7f-f82f-4a6e-9900-bfb368e13abe2023-06-05T14:26:07ZTools for decoding ubiquitin signaling in DNA repairJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a728ca7f-f82f-4a6e-9900-bfb368e13abeEnglishSymplectic ElementsFrontiers Media2021Foster, BAttwood, MGibbs-Seymour, IThe maintenance of genome stability requires dedicated DNA repair processes and pathways that are essential for the faithful duplication and propagation of chromosomes. These DNA repair mechanisms counteract the potentially deleterious impact of the frequent genotoxic challenges faced by cells from both exogenous and endogenous agents. Intrinsic to these mechanisms, cells have an arsenal of protein factors that can be utilised to promote repair processes in response to DNA lesions. Orchestration of the protein factors within the various cellular DNA repair pathways is performed, in part, by post-translational modifications, such as phosphorylation, ubiquitin, SUMO and other ubiquitin-like modifiers (UBLs). In this review, we firstly explore recent advances in the tools for identifying factors involved in both DNA repair and ubiquitin signaling pathways. We then expand on this by evaluating the growing repertoire of proteomic, biochemical and structural techniques available to further understand the mechanistic basis by which these complex modifications regulate DNA repair. Together, we provide a snapshot of the range of methods now available to investigate and decode how ubiquitin signaling can promote DNA repair and maintain genome stability in mammalian cells. |
| spellingShingle | Foster, B Attwood, M Gibbs-Seymour, I Tools for decoding ubiquitin signaling in DNA repair |
| title | Tools for decoding ubiquitin signaling in DNA repair |
| title_full | Tools for decoding ubiquitin signaling in DNA repair |
| title_fullStr | Tools for decoding ubiquitin signaling in DNA repair |
| title_full_unstemmed | Tools for decoding ubiquitin signaling in DNA repair |
| title_short | Tools for decoding ubiquitin signaling in DNA repair |
| title_sort | tools for decoding ubiquitin signaling in dna repair |
| work_keys_str_mv | AT fosterb toolsfordecodingubiquitinsignalingindnarepair AT attwoodm toolsfordecodingubiquitinsignalingindnarepair AT gibbsseymouri toolsfordecodingubiquitinsignalingindnarepair |