Cutting edge: quantitative imaging of raft accumulation in the immunological synapse.
Although the accumulation of lipid rafts at the immunological synapse is now well accepted, the degree of the accumulation, the localization within the fine structure of the immunological synapse, and the region from which lipid rafts are recruited have not been defined. In this work we show that li...
| প্রধান লেখক: | , , , , |
|---|---|
| বিন্যাস: | Journal article |
| ভাষা: | English |
| প্রকাশিত: |
2002
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| _version_ | 1826289657539723264 |
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| author | Burack, W Lee, K Holdorf, A Dustin, M Shaw, A |
| author_facet | Burack, W Lee, K Holdorf, A Dustin, M Shaw, A |
| author_sort | Burack, W |
| collection | OXFORD |
| description | Although the accumulation of lipid rafts at the immunological synapse is now well accepted, the degree of the accumulation, the localization within the fine structure of the immunological synapse, and the region from which lipid rafts are recruited have not been defined. In this work we show that lipid rafts preferentially accumulate in the central zone of the immunological synapse, the central supramolecular activation complex (C-SMAC). However, quantitative analyses indicate that the level of recruitment of lipid rafts to the C-SMAC is relatively small and suggests that rearrangement of lipid rafts from the peripheral zone of the synapse into the C-SMAC can account for this accumulation. We also assessed the effects of CD28 deficiency on lipid raft recruitment to the immunological synapse. The accumulation of lipid occurred independently of the CD28/B7 system and was not measurably altered by CD28. |
| first_indexed | 2024-03-07T02:32:14Z |
| format | Journal article |
| id | oxford-uuid:a7a08b60-d1d2-453e-b43f-53fb4fdaaba0 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:32:14Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:a7a08b60-d1d2-453e-b43f-53fb4fdaaba02022-03-27T02:55:53ZCutting edge: quantitative imaging of raft accumulation in the immunological synapse.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a7a08b60-d1d2-453e-b43f-53fb4fdaaba0EnglishSymplectic Elements at Oxford2002Burack, WLee, KHoldorf, ADustin, MShaw, AAlthough the accumulation of lipid rafts at the immunological synapse is now well accepted, the degree of the accumulation, the localization within the fine structure of the immunological synapse, and the region from which lipid rafts are recruited have not been defined. In this work we show that lipid rafts preferentially accumulate in the central zone of the immunological synapse, the central supramolecular activation complex (C-SMAC). However, quantitative analyses indicate that the level of recruitment of lipid rafts to the C-SMAC is relatively small and suggests that rearrangement of lipid rafts from the peripheral zone of the synapse into the C-SMAC can account for this accumulation. We also assessed the effects of CD28 deficiency on lipid raft recruitment to the immunological synapse. The accumulation of lipid occurred independently of the CD28/B7 system and was not measurably altered by CD28. |
| spellingShingle | Burack, W Lee, K Holdorf, A Dustin, M Shaw, A Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title | Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title_full | Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title_fullStr | Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title_full_unstemmed | Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title_short | Cutting edge: quantitative imaging of raft accumulation in the immunological synapse. |
| title_sort | cutting edge quantitative imaging of raft accumulation in the immunological synapse |
| work_keys_str_mv | AT burackw cuttingedgequantitativeimagingofraftaccumulationintheimmunologicalsynapse AT leek cuttingedgequantitativeimagingofraftaccumulationintheimmunologicalsynapse AT holdorfa cuttingedgequantitativeimagingofraftaccumulationintheimmunologicalsynapse AT dustinm cuttingedgequantitativeimagingofraftaccumulationintheimmunologicalsynapse AT shawa cuttingedgequantitativeimagingofraftaccumulationintheimmunologicalsynapse |