Auto-aligning stimulated emission depletion microscope using adaptive optics.
Stimulated emission depletion (STED) microscopy provides diffraction-unlimited resolution in fluorescence microscopy. Imaging at the nanoscale, however, requires precise alignment of the depletion and excitation laser foci of the STED microscope. We demonstrate here that adaptive optics can be imple...
Main Authors: | Gould, T, Kromann, E, Burke, D, Booth, M, Bewersdorf, J |
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Format: | Journal article |
Language: | English |
Published: |
2013
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