Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease.
BACKGROUND and AIMS: Crohn's disease (CD) is associated with nutritional deficiencies, altered plasma concentrations of polyunsaturated fatty acids (PUFA) and an anti-inflammatory response to fish oil that contains n-3 PUFA. This suggests that, in CD, immune cells may have altered n-3 PUFA com...
| Main Authors: | , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1797087282570723328 |
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| author | Trebble, T Arden, N Wootton, SA Mullee, M Calder, P Burdge, G Fine, DR Stroud, M |
| author_facet | Trebble, T Arden, N Wootton, SA Mullee, M Calder, P Burdge, G Fine, DR Stroud, M |
| author_sort | Trebble, T |
| collection | OXFORD |
| description | BACKGROUND and AIMS: Crohn's disease (CD) is associated with nutritional deficiencies, altered plasma concentrations of polyunsaturated fatty acids (PUFA) and an anti-inflammatory response to fish oil that contains n-3 PUFA. This suggests that, in CD, immune cells may have altered n-3 PUFA composition with functional consequences. The aim of this study is to investigate n-3 and n-6 PUFA composition and synthetic function of peripheral blood mononuclear cells (PBMC) in the basal state. METHODS: A case control study of 52 adult CD patients and healthy, age- and sex-matched controls. Composition of PBMC and plasma phospholipids were measured by gas chromatography and production of tumour necrosis factor-alpha, prostaglandin E2 (PGE2) and interferon-gamma (IFN-gamma) by PBMC were measured by ELISA. RESULTS: CD was associated with higher concentrations of eicosapentaenoic acid and other n-3 PUFA, and lower arachidonic acid (AA) (n-6 PUFA) in PBMC. This was not explained by differences in dietary fat intake. Lower rates of production of PGE2 and IFN-gamma by PBMC were noted in quiescent and active CD, respectively, compared to controls. CONCLUSIONS: CD is associated with a greater availability, and not a deficiency, of n-3 PUFA in PBMC, but lower concentrations of AA, and lower rates of production of PGE2 and IFN-gamma, compared to healthy controls. |
| first_indexed | 2024-03-07T02:33:33Z |
| format | Journal article |
| id | oxford-uuid:a8098d22-dd69-4010-96d8-1632bc783e72 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:33:33Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:a8098d22-dd69-4010-96d8-1632bc783e722022-03-27T02:58:40ZPeripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a8098d22-dd69-4010-96d8-1632bc783e72EnglishSymplectic Elements at Oxford2004Trebble, TArden, NWootton, SAMullee, MCalder, PBurdge, GFine, DRStroud, M BACKGROUND and AIMS: Crohn's disease (CD) is associated with nutritional deficiencies, altered plasma concentrations of polyunsaturated fatty acids (PUFA) and an anti-inflammatory response to fish oil that contains n-3 PUFA. This suggests that, in CD, immune cells may have altered n-3 PUFA composition with functional consequences. The aim of this study is to investigate n-3 and n-6 PUFA composition and synthetic function of peripheral blood mononuclear cells (PBMC) in the basal state. METHODS: A case control study of 52 adult CD patients and healthy, age- and sex-matched controls. Composition of PBMC and plasma phospholipids were measured by gas chromatography and production of tumour necrosis factor-alpha, prostaglandin E2 (PGE2) and interferon-gamma (IFN-gamma) by PBMC were measured by ELISA. RESULTS: CD was associated with higher concentrations of eicosapentaenoic acid and other n-3 PUFA, and lower arachidonic acid (AA) (n-6 PUFA) in PBMC. This was not explained by differences in dietary fat intake. Lower rates of production of PGE2 and IFN-gamma by PBMC were noted in quiescent and active CD, respectively, compared to controls. CONCLUSIONS: CD is associated with a greater availability, and not a deficiency, of n-3 PUFA in PBMC, but lower concentrations of AA, and lower rates of production of PGE2 and IFN-gamma, compared to healthy controls. |
| spellingShingle | Trebble, T Arden, N Wootton, SA Mullee, M Calder, P Burdge, G Fine, DR Stroud, M Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title | Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title_full | Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title_fullStr | Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title_full_unstemmed | Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title_short | Peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult Crohn's disease. |
| title_sort | peripheral blood mononuclear cell fatty acid composition and inflammatory mediator production in adult crohn s disease |
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