Applications of stable isotope dimethyl labeling in quantitative proteomics.
Mass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2012
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| _version_ | 1826289889137655808 |
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| author | Kovanich, D Cappadona, S Raijmakers, R Mohammed, S Scholten, A Heck, A |
| author_facet | Kovanich, D Cappadona, S Raijmakers, R Mohammed, S Scholten, A Heck, A |
| author_sort | Kovanich, D |
| collection | OXFORD |
| description | Mass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able to compete at any level with the present alternatives. In this review, we briefly introduce experimental and software methods for proteome analysis using dimethyl labeling and provide a comprehensive overview of reported applications in the analysis of (1) differential protein expression, (2) posttranslational modifications, and (3) protein interactions. |
| first_indexed | 2024-03-07T02:35:47Z |
| format | Journal article |
| id | oxford-uuid:a8bdad2a-3bff-44ad-97b4-902deb533dc5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:35:47Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:a8bdad2a-3bff-44ad-97b4-902deb533dc52022-03-27T03:03:38ZApplications of stable isotope dimethyl labeling in quantitative proteomics.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a8bdad2a-3bff-44ad-97b4-902deb533dc5EnglishSymplectic Elements at Oxford2012Kovanich, DCappadona, SRaijmakers, RMohammed, SScholten, AHeck, AMass spectrometry has proven to be an indispensable tool for protein identification, characterization, and quantification. Among the possible methods in quantitative proteomics, stable isotope labeling by using reductive dimethylation has emerged as a cost-effective, simple, but powerful method able to compete at any level with the present alternatives. In this review, we briefly introduce experimental and software methods for proteome analysis using dimethyl labeling and provide a comprehensive overview of reported applications in the analysis of (1) differential protein expression, (2) posttranslational modifications, and (3) protein interactions. |
| spellingShingle | Kovanich, D Cappadona, S Raijmakers, R Mohammed, S Scholten, A Heck, A Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title | Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title_full | Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title_fullStr | Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title_full_unstemmed | Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title_short | Applications of stable isotope dimethyl labeling in quantitative proteomics. |
| title_sort | applications of stable isotope dimethyl labeling in quantitative proteomics |
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