Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.
The monoclonal antibody UCHL1 identified an antigen present on most thymocytes, a subpopulation of resting T cells within both the CD4 and CD8 subsets, and on mature activated T cells. The UCHL1 determinant is also present on cells of the myeloid lineage, but not normal B cells or NK cells. Function...
| Hoofdauteurs: | , , , , |
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| Formaat: | Journal article |
| Taal: | English |
| Gepubliceerd in: |
1986
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| _version_ | 1826289920065404928 |
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| author | Smith, S Brown, M Rowe, D Callard, R Beverley, P |
| author_facet | Smith, S Brown, M Rowe, D Callard, R Beverley, P |
| author_sort | Smith, S |
| collection | OXFORD |
| description | The monoclonal antibody UCHL1 identified an antigen present on most thymocytes, a subpopulation of resting T cells within both the CD4 and CD8 subsets, and on mature activated T cells. The UCHL1 determinant is also present on cells of the myeloid lineage, but not normal B cells or NK cells. Functionally, UCHL1 identifies a subpopulation of T cells which proliferates maximally to soluble antigen and provides maximum help for PWM-stimulated immunoglobulin synthesis. In contrast, the UCHL1- cells do not induce immunoglobulin synthesis and do not proliferate in the presence of soluble antigen, although both the UCHL1- and the UCHL1+ fractions of T cells proliferate well in the presence of PHA. By standard immunoprecipitation techniques and SDS page, the antigen recognized by UCHL1 was found to have a molecular weight of 180,000-185,000. Preclearing experiments using antibodies identifying the leucocyte common antigen, LCA, and the lymphocyte function-associated antigen, LFA-1, which have similar molecular weights to UCHL1, showed that the UCHL1 determinant is not biochemically related to these antigens. |
| first_indexed | 2024-03-07T02:36:16Z |
| format | Journal article |
| id | oxford-uuid:a8e4fc7c-b074-4c5c-86b4-cc32ec70eeee |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:36:16Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:a8e4fc7c-b074-4c5c-86b4-cc32ec70eeee2022-03-27T03:04:47ZFunctional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a8e4fc7c-b074-4c5c-86b4-cc32ec70eeeeEnglishSymplectic Elements at Oxford1986Smith, SBrown, MRowe, DCallard, RBeverley, PThe monoclonal antibody UCHL1 identified an antigen present on most thymocytes, a subpopulation of resting T cells within both the CD4 and CD8 subsets, and on mature activated T cells. The UCHL1 determinant is also present on cells of the myeloid lineage, but not normal B cells or NK cells. Functionally, UCHL1 identifies a subpopulation of T cells which proliferates maximally to soluble antigen and provides maximum help for PWM-stimulated immunoglobulin synthesis. In contrast, the UCHL1- cells do not induce immunoglobulin synthesis and do not proliferate in the presence of soluble antigen, although both the UCHL1- and the UCHL1+ fractions of T cells proliferate well in the presence of PHA. By standard immunoprecipitation techniques and SDS page, the antigen recognized by UCHL1 was found to have a molecular weight of 180,000-185,000. Preclearing experiments using antibodies identifying the leucocyte common antigen, LCA, and the lymphocyte function-associated antigen, LFA-1, which have similar molecular weights to UCHL1, showed that the UCHL1 determinant is not biochemically related to these antigens. |
| spellingShingle | Smith, S Brown, M Rowe, D Callard, R Beverley, P Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title | Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title_full | Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title_fullStr | Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title_full_unstemmed | Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title_short | Functional subsets of human helper-inducer cells defined by a new monoclonal antibody, UCHL1. |
| title_sort | functional subsets of human helper inducer cells defined by a new monoclonal antibody uchl1 |
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