Different mechanisms underlie the long‐term regulation of pyruvate dehydrogenase kinase (PDHK) by tri‐iodothyronine in heart and liver

Antibodies to purified recombinant PDHKII were used for ELISAs of PDHKII in mitochondrial extracts. In liver, hyperthyroidism elicited a 2.3‐fold increase in PDHK activity (P<0.01) which was accompanied by a significant 1.5‐fold (P<0.001) increase in the amount of mitochondrial immunor...

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Hlavní autoři: Priestman, D, Donald, E, Holness, M, al., E
Médium: Journal article
Vydáno: Wiley 1997
Popis
Shrnutí:Antibodies to purified recombinant PDHKII were used for ELISAs of PDHKII in mitochondrial extracts. In liver, hyperthyroidism elicited a 2.3‐fold increase in PDHK activity (P<0.01) which was accompanied by a significant 1.5‐fold (P<0.001) increase in the amount of mitochondrial immunoreactive PDHKII. In contrast, despite a stable 2.0‐fold increase in cardiac PDHK activity (P<0.001), the amount of mitochondrial immunoreactive PDHKII in heart was unaffected by hyperthyroidism. The mechanisms for long‐term regulation of PDHK activity by thyroid hormones therefore differ fundamentally between heart and liver.