Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics
The analysis of cargo proteins in exosome subpopulations has considerable value in diagnostics but a translatable impact has been limited by lengthy or complex exosome extraction protocols. We describe herein a scalable, fast, and low-cost exosome extraction using an alternating (AC) magnetic field...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
American Chemical Society
2023
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| _version_ | 1797110438458032128 |
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| author | Sharafeldin, M Yan, S Jiang, C Tofaris, GK Davis, JJ |
| author_facet | Sharafeldin, M Yan, S Jiang, C Tofaris, GK Davis, JJ |
| author_sort | Sharafeldin, M |
| collection | OXFORD |
| description | The analysis of cargo proteins in exosome subpopulations has considerable value in diagnostics but a translatable impact has been limited by lengthy or complex exosome extraction protocols. We describe herein a scalable, fast, and low-cost exosome extraction using an alternating (AC) magnetic field to support the dynamic mixing of antibody-coated magnetic beads (MBs) with serum samples within 3D-printed microfluidic chips. Zwitterionic polymer-coated MBs are, specifically, magnetically agitated and support ultraclean exosome capture efficiencies >70% from <50 μL of neat serum in 30 min. Applied herein to the immunocapture of neuronal exosomes using anti-L1CAM antibodies, prior to the array-based assaying of α-synuclein (α-syn) content by a standard duplex electrochemical sandwich ELISA, sub pg/mL detection was possible with an excellent coefficient of variation and a sample-to-answer time of ∼75 min. The high performance and semiautomation of this approach hold promise in underpinning low-cost Parkinson’s disease diagnostics and is of value in exosomal biomarker analyses more generally. |
| first_indexed | 2024-03-07T07:54:51Z |
| format | Journal article |
| id | oxford-uuid:a9a20f6f-6bed-4aba-9164-67c32fac1259 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T07:54:51Z |
| publishDate | 2023 |
| publisher | American Chemical Society |
| record_format | dspace |
| spelling | oxford-uuid:a9a20f6f-6bed-4aba-9164-67c32fac12592023-08-11T11:10:24ZAlternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnosticsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a9a20f6f-6bed-4aba-9164-67c32fac1259EnglishSymplectic ElementsAmerican Chemical Society2023Sharafeldin, MYan, SJiang, CTofaris, GKDavis, JJThe analysis of cargo proteins in exosome subpopulations has considerable value in diagnostics but a translatable impact has been limited by lengthy or complex exosome extraction protocols. We describe herein a scalable, fast, and low-cost exosome extraction using an alternating (AC) magnetic field to support the dynamic mixing of antibody-coated magnetic beads (MBs) with serum samples within 3D-printed microfluidic chips. Zwitterionic polymer-coated MBs are, specifically, magnetically agitated and support ultraclean exosome capture efficiencies >70% from <50 μL of neat serum in 30 min. Applied herein to the immunocapture of neuronal exosomes using anti-L1CAM antibodies, prior to the array-based assaying of α-synuclein (α-syn) content by a standard duplex electrochemical sandwich ELISA, sub pg/mL detection was possible with an excellent coefficient of variation and a sample-to-answer time of ∼75 min. The high performance and semiautomation of this approach hold promise in underpinning low-cost Parkinson’s disease diagnostics and is of value in exosomal biomarker analyses more generally. |
| spellingShingle | Sharafeldin, M Yan, S Jiang, C Tofaris, GK Davis, JJ Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title | Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title_full | Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title_fullStr | Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title_full_unstemmed | Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title_short | Alternating magnetic field-promoted nanoparticle mixing: the on-chip immunocapture of serum neuronal exosomes for Parkinson’s disease diagnostics |
| title_sort | alternating magnetic field promoted nanoparticle mixing the on chip immunocapture of serum neuronal exosomes for parkinson s disease diagnostics |
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