The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage
Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing <i>Esche...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
Microbiology Society
2023
|
| _version_ | 1797111307022893056 |
|---|---|
| author | Snaith, AE Dunn, SJ Moran, RA Newton, PN Dance, DAB Davong, V Kuenzli, E Kantele, A Corander, J McNally, A |
| author_facet | Snaith, AE Dunn, SJ Moran, RA Newton, PN Dance, DAB Davong, V Kuenzli, E Kantele, A Corander, J McNally, A |
| author_sort | Snaith, AE |
| collection | OXFORD |
| description | Increased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> that colonized travellers to Laos. Long-read sequencing was used to reconstruct complete plasmid sequences from 48 isolates obtained from the daily stool samples of 23 travellers over a 3 week period. This method revealed a collection of 105 distinct plasmids, 38.1 % (<i>n</i>=40) of which carried AMR genes. The plasmids in this population were diverse, mostly unreported and included 38 replicon types, with F-type plasmids (<i>n</i>=23) the most prevalent amongst those carrying AMR genes. Fine-scale analysis of all plasmids identified numerous AMR gene contexts and emphasized the importance of IS elements, specifically members of the IS<i>6</i>/IS<i>26</i> family, in the evolution of complex multidrug resistance regions. We found a concerning convergence of ESBL and colistin resistance determinants, with three plasmids from two different F-type lineages carrying <i>bla</i><sub>CTX-M</sub> and <i>mcr</i> genes. The extensive diversity seen here highlights the worrying probability that stable new vehicles for AMR will evolve in <i>E. coli</i> populations that can disseminate internationally through travel networks. |
| first_indexed | 2024-03-07T08:08:29Z |
| format | Journal article |
| id | oxford-uuid:a9fd5170-5c63-4ea0-a773-7c2b7b5920cf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:08:29Z |
| publishDate | 2023 |
| publisher | Microbiology Society |
| record_format | dspace |
| spelling | oxford-uuid:a9fd5170-5c63-4ea0-a773-7c2b7b5920cf2023-11-08T14:41:53ZThe highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriageJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:a9fd5170-5c63-4ea0-a773-7c2b7b5920cfEnglishSymplectic ElementsMicrobiology Society2023Snaith, AEDunn, SJMoran, RANewton, PNDance, DABDavong, VKuenzli, EKantele, ACorander, JMcNally, AIncreased colonization by antimicrobial-resistant organisms is closely associated with international travel. This study investigated the diversity of mobile genetic elements involved with antimicrobial resistance (AMR) gene carriage in extended-spectrum beta-lactamase (ESBL)-producing <i>Escherichia coli</i> that colonized travellers to Laos. Long-read sequencing was used to reconstruct complete plasmid sequences from 48 isolates obtained from the daily stool samples of 23 travellers over a 3 week period. This method revealed a collection of 105 distinct plasmids, 38.1 % (<i>n</i>=40) of which carried AMR genes. The plasmids in this population were diverse, mostly unreported and included 38 replicon types, with F-type plasmids (<i>n</i>=23) the most prevalent amongst those carrying AMR genes. Fine-scale analysis of all plasmids identified numerous AMR gene contexts and emphasized the importance of IS elements, specifically members of the IS<i>6</i>/IS<i>26</i> family, in the evolution of complex multidrug resistance regions. We found a concerning convergence of ESBL and colistin resistance determinants, with three plasmids from two different F-type lineages carrying <i>bla</i><sub>CTX-M</sub> and <i>mcr</i> genes. The extensive diversity seen here highlights the worrying probability that stable new vehicles for AMR will evolve in <i>E. coli</i> populations that can disseminate internationally through travel networks. |
| spellingShingle | Snaith, AE Dunn, SJ Moran, RA Newton, PN Dance, DAB Davong, V Kuenzli, E Kantele, A Corander, J McNally, A The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title | The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title_full | The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title_fullStr | The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title_full_unstemmed | The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title_short | The highly diverse plasmid population found in Escherichia coli colonizing travellers to Laos and its role in antimicrobial resistance gene carriage |
| title_sort | highly diverse plasmid population found in escherichia coli colonizing travellers to laos and its role in antimicrobial resistance gene carriage |
| work_keys_str_mv | AT snaithae thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT dunnsj thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT moranra thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT newtonpn thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT dancedab thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT davongv thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT kuenzlie thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT kantelea thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT coranderj thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT mcnallya thehighlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT snaithae highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT dunnsj highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT moranra highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT newtonpn highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT dancedab highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT davongv highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT kuenzlie highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT kantelea highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT coranderj highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage AT mcnallya highlydiverseplasmidpopulationfoundinescherichiacolicolonizingtravellerstolaosanditsroleinantimicrobialresistancegenecarriage |