Syncoilin isoform organization and differential expression in murine striated muscle.
Syncoilin is a 64kDa intermediate filament (IF) protein expressed in myocytes at the sarcolemma, perinucleus, myotendenous and neuromuscular junctions. Here we present a revised domain projection and structural analysis for the original isoform (sync-1) and introduce two novel syncoilin isoforms (sy...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2009
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| _version_ | 1826290178496397312 |
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| author | Kemp, M Edwards, B Burgess, M Clarke, W Nicholson, G Parry, D Davies, K |
| author_facet | Kemp, M Edwards, B Burgess, M Clarke, W Nicholson, G Parry, D Davies, K |
| author_sort | Kemp, M |
| collection | OXFORD |
| description | Syncoilin is a 64kDa intermediate filament (IF) protein expressed in myocytes at the sarcolemma, perinucleus, myotendenous and neuromuscular junctions. Here we present a revised domain projection and structural analysis for the original isoform (sync-1) and introduce two novel syncoilin isoforms (sync-2 and sync-3) generated by exon splicing. On the basis of consensus identity we propose that syncoilin be reclassified as a type III IF protein. All three syncoilin isoforms lack a L1 domain, a significant departure from standard IF rod domain projections that is likely to impact significantly on their biological function. Our analyses indicate that syncoilin is unlikely to form classical intermediate filament structures by itself, and that the significant difference in C-terminal structure between the three isoforms indicates that they may play divergent roles in myocytes. We show that despite lacking an apparent structural role in striated muscle, syncoilin isoforms are differentially and strongly upregulated in response to cardiotoxin induced regeneration and denervation induced atrophy in the C57BL/6 mouse, possibly suggesting an atypical role for syncoilin in muscle. |
| first_indexed | 2024-03-07T02:40:13Z |
| format | Journal article |
| id | oxford-uuid:aa2f9559-9f42-4ed8-a264-da690c8bb399 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:40:13Z |
| publishDate | 2009 |
| record_format | dspace |
| spelling | oxford-uuid:aa2f9559-9f42-4ed8-a264-da690c8bb3992022-03-27T03:13:29ZSyncoilin isoform organization and differential expression in murine striated muscle.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:aa2f9559-9f42-4ed8-a264-da690c8bb399EnglishSymplectic Elements at Oxford2009Kemp, MEdwards, BBurgess, MClarke, WNicholson, GParry, DDavies, KSyncoilin is a 64kDa intermediate filament (IF) protein expressed in myocytes at the sarcolemma, perinucleus, myotendenous and neuromuscular junctions. Here we present a revised domain projection and structural analysis for the original isoform (sync-1) and introduce two novel syncoilin isoforms (sync-2 and sync-3) generated by exon splicing. On the basis of consensus identity we propose that syncoilin be reclassified as a type III IF protein. All three syncoilin isoforms lack a L1 domain, a significant departure from standard IF rod domain projections that is likely to impact significantly on their biological function. Our analyses indicate that syncoilin is unlikely to form classical intermediate filament structures by itself, and that the significant difference in C-terminal structure between the three isoforms indicates that they may play divergent roles in myocytes. We show that despite lacking an apparent structural role in striated muscle, syncoilin isoforms are differentially and strongly upregulated in response to cardiotoxin induced regeneration and denervation induced atrophy in the C57BL/6 mouse, possibly suggesting an atypical role for syncoilin in muscle. |
| spellingShingle | Kemp, M Edwards, B Burgess, M Clarke, W Nicholson, G Parry, D Davies, K Syncoilin isoform organization and differential expression in murine striated muscle. |
| title | Syncoilin isoform organization and differential expression in murine striated muscle. |
| title_full | Syncoilin isoform organization and differential expression in murine striated muscle. |
| title_fullStr | Syncoilin isoform organization and differential expression in murine striated muscle. |
| title_full_unstemmed | Syncoilin isoform organization and differential expression in murine striated muscle. |
| title_short | Syncoilin isoform organization and differential expression in murine striated muscle. |
| title_sort | syncoilin isoform organization and differential expression in murine striated muscle |
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