The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.
A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Hollid...
| Main Authors: | , , |
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| Format: | Journal article |
| Language: | English |
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2010
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| _version_ | 1826290218743889920 |
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| author | Lorenz, A West, S Whitby, M |
| author_facet | Lorenz, A West, S Whitby, M |
| author_sort | Lorenz, A |
| collection | OXFORD |
| description | A key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation during meiosis. |
| first_indexed | 2024-03-07T02:40:49Z |
| format | Journal article |
| id | oxford-uuid:aa64d273-789b-456f-bd29-1ddde5ba6182 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:40:49Z |
| publishDate | 2010 |
| record_format | dspace |
| spelling | oxford-uuid:aa64d273-789b-456f-bd29-1ddde5ba61822022-03-27T03:14:42ZThe human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:aa64d273-789b-456f-bd29-1ddde5ba6182EnglishSymplectic Elements at Oxford2010Lorenz, AWest, SWhitby, MA key step in meiotic recombination involves the nucleolytic resolution of Holliday junctions to generate crossovers. Although the enzyme that performs this function in human cells is presently unknown, recent studies led to the identification of the XPG-family endonuclease GEN1 that promotes Holliday junction resolution in vitro, suggesting that it may perform a related function in vivo. Here, we show that ectopic expression of GEN1 in fission yeast mus81Delta strains results in Holliday junction resolution and crossover formation during meiosis. |
| spellingShingle | Lorenz, A West, S Whitby, M The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title_full | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title_fullStr | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title_full_unstemmed | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title_short | The human Holliday junction resolvase GEN1 rescues the meiotic phenotype of a Schizosaccharomyces pombe mus81 mutant. |
| title_sort | human holliday junction resolvase gen1 rescues the meiotic phenotype of a schizosaccharomyces pombe mus81 mutant |
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