Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.
A system devised for tissue typing the HLA-A locus by PCR from genomic DNA has been used to investigate abnormalities of HLA expression in a panel of 30 colorectal tumor cell lines, by comparing the HLA-A locus genotype with surface expression of HLA. Three cell lines showed complete lack of HLA exp...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1993
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| _version_ | 1826290319851782144 |
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| author | Browning, M Krausa, P Rowan, A Bicknell, D Bodmer, J Bodmer, W |
| author_facet | Browning, M Krausa, P Rowan, A Bicknell, D Bodmer, J Bodmer, W |
| author_sort | Browning, M |
| collection | OXFORD |
| description | A system devised for tissue typing the HLA-A locus by PCR from genomic DNA has been used to investigate abnormalities of HLA expression in a panel of 30 colorectal tumor cell lines, by comparing the HLA-A locus genotype with surface expression of HLA. Three cell lines showed complete lack of HLA expression associated with failure to express beta 2-microglobulin. In two other cell lines, loss of expression of HLA-A2 was observed, in spite of the presence of the gene in genomic DNA. Eleven cell lines gave a single HLA-A locus specificity on PCR typing. In one of these cell lines we have demonstrated the loss of an HLA-A locus gene in the tumor cell by comparison with DNA from a lymphoblastoid B-cell line derived from the same patient. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines. |
| first_indexed | 2024-03-07T02:42:22Z |
| format | Journal article |
| id | oxford-uuid:aae3cc56-a46d-4d01-b74a-b7a4271abd5a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:42:22Z |
| publishDate | 1993 |
| record_format | dspace |
| spelling | oxford-uuid:aae3cc56-a46d-4d01-b74a-b7a4271abd5a2022-03-27T03:18:13ZTissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:aae3cc56-a46d-4d01-b74a-b7a4271abd5aEnglishSymplectic Elements at Oxford1993Browning, MKrausa, PRowan, ABicknell, DBodmer, JBodmer, WA system devised for tissue typing the HLA-A locus by PCR from genomic DNA has been used to investigate abnormalities of HLA expression in a panel of 30 colorectal tumor cell lines, by comparing the HLA-A locus genotype with surface expression of HLA. Three cell lines showed complete lack of HLA expression associated with failure to express beta 2-microglobulin. In two other cell lines, loss of expression of HLA-A2 was observed, in spite of the presence of the gene in genomic DNA. Eleven cell lines gave a single HLA-A locus specificity on PCR typing. In one of these cell lines we have demonstrated the loss of an HLA-A locus gene in the tumor cell by comparison with DNA from a lymphoblastoid B-cell line derived from the same patient. These data indicate that at least three independent mechanisms were involved in the loss of HLA expression on the colorectal tumor cell lines. |
| spellingShingle | Browning, M Krausa, P Rowan, A Bicknell, D Bodmer, J Bodmer, W Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title | Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title_full | Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title_fullStr | Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title_full_unstemmed | Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title_short | Tissue typing the HLA-A locus from genomic DNA by sequence-specific PCR: comparison of HLA genotype and surface expression on colorectal tumor cell lines. |
| title_sort | tissue typing the hla a locus from genomic dna by sequence specific pcr comparison of hla genotype and surface expression on colorectal tumor cell lines |
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