The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells
<p style="text-align:justify;"> Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protec...
| Автори: | , , , , , |
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| Формат: | Journal article |
| Мова: | English |
| Опубліковано: |
Wiley
2010
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| _version_ | 1826290378666409984 |
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| author | Xu, X Cowley, S Flaim, C James, W Seymour, L Cui, Z |
| author_facet | Xu, X Cowley, S Flaim, C James, W Seymour, L Cui, Z |
| author_sort | Xu, X |
| collection | OXFORD |
| description | <p style="text-align:justify;"> Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing efficient and successful cryopreservation methods for hES cell lines and banks. We investigated how the stimuli from cryopreservation result in apoptosis, which causes the low cell recovery rate after cryopreservation. The level of reactive oxygen species (ROS) is significantly increased, F‐actin content and distribution is altered, and caspase‐8 and caspase‐9 are activated after cryopreservation. p53 is also activated and translocated into nucleus. During cryopreservation apoptosis is induced by activation of both caspase‐8 through the extrinsic pathway and caspase‐9 through the intrinsic pathway. However, exactly how the extrinsic pathway is activated is still unclear and deserves further investigation. </p> |
| first_indexed | 2024-03-07T02:43:17Z |
| format | Journal article |
| id | oxford-uuid:ab2d0064-e084-418c-90f2-dfcdf8ef6f33 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:43:17Z |
| publishDate | 2010 |
| publisher | Wiley |
| record_format | dspace |
| spelling | oxford-uuid:ab2d0064-e084-418c-90f2-dfcdf8ef6f332022-03-27T03:20:14ZThe roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cellsJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ab2d0064-e084-418c-90f2-dfcdf8ef6f33EnglishSymplectic Elements at OxfordWiley2010Xu, XCowley, SFlaim, CJames, WSeymour, LCui, Z <p style="text-align:justify;"> Human embryonic stem (hES) cells have enormous potential for clinical applications. However, one major challenge is to achieve high cell recovery rate after cryopreservation. Understanding how the conventional cryopreservation protocol fails to protect the cells is a prerequisite for developing efficient and successful cryopreservation methods for hES cell lines and banks. We investigated how the stimuli from cryopreservation result in apoptosis, which causes the low cell recovery rate after cryopreservation. The level of reactive oxygen species (ROS) is significantly increased, F‐actin content and distribution is altered, and caspase‐8 and caspase‐9 are activated after cryopreservation. p53 is also activated and translocated into nucleus. During cryopreservation apoptosis is induced by activation of both caspase‐8 through the extrinsic pathway and caspase‐9 through the intrinsic pathway. However, exactly how the extrinsic pathway is activated is still unclear and deserves further investigation. </p> |
| spellingShingle | Xu, X Cowley, S Flaim, C James, W Seymour, L Cui, Z The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title | The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title_full | The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title_fullStr | The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title_full_unstemmed | The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title_short | The roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
| title_sort | roles of apoptotic pathways in the low recovery rate after cryopreservation of dissociated human embryonic stem cells |
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