Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.

Straightforward ladder sequencing of peptides using a Lys-N metalloendopeptidase.

We introduce a method for sequencing peptides by mass spectrometry using a metalloendopeptidase that cleaves proteins at the amino side of lysine (Lys-N). When analyzed by electron transfer dissociation (ETD)-based mass spectrometric sequencing, Lys-N-digested peptides that contain a single lysine r...

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Bibliographic Details
Main Authors:	Taouatas, N, Drugan, M, Heck, A, Mohammed, S
Format:	Journal article
Language:	English
Published:	2008

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