Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison.
The MHC class I pathway is usually fueled by endogenous Ags, while exogenous Ags reach the MHC class II pathway. Although exogenous epitopes may also enter the MHC class I pathway, quantification of the efficiency of the process has remained a difficult task. In an attempt of such a quantification,...
| Main Authors: | , |
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| Format: | Journal article |
| Language: | English |
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2004
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| _version_ | 1826290407227523072 |
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| author | Storni, T Bachmann, M |
| author_facet | Storni, T Bachmann, M |
| author_sort | Storni, T |
| collection | OXFORD |
| description | The MHC class I pathway is usually fueled by endogenous Ags, while exogenous Ags reach the MHC class II pathway. Although exogenous epitopes may also enter the MHC class I pathway, quantification of the efficiency of the process has remained a difficult task. In an attempt of such a quantification, we directly compared the amount of exogenous virus-like particles required for induction of cytotoxic T cell responses by cross-priming with the amount of virus-like particles required for induction of Th cell responses by the conventional route of MHC class II loading as an internal standard. Surprisingly, we found that cross-presentation of peptides derived from exogenous Ags on MHC class I molecules is of only marginally lower efficiency ( approximately 1- to 10-fold) than the classical MHC class II pathway in vitro and in vivo. Thus, Ag quantities required for cross-presentation and cross-priming are similar to those required for fueling the MHC class II pathway. |
| first_indexed | 2024-03-07T02:43:43Z |
| format | Journal article |
| id | oxford-uuid:ab4ffed7-0147-467e-b619-aa951b7fb4b5 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:43:43Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:ab4ffed7-0147-467e-b619-aa951b7fb4b52022-03-27T03:21:07ZLoading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ab4ffed7-0147-467e-b619-aa951b7fb4b5EnglishSymplectic Elements at Oxford2004Storni, TBachmann, MThe MHC class I pathway is usually fueled by endogenous Ags, while exogenous Ags reach the MHC class II pathway. Although exogenous epitopes may also enter the MHC class I pathway, quantification of the efficiency of the process has remained a difficult task. In an attempt of such a quantification, we directly compared the amount of exogenous virus-like particles required for induction of cytotoxic T cell responses by cross-priming with the amount of virus-like particles required for induction of Th cell responses by the conventional route of MHC class II loading as an internal standard. Surprisingly, we found that cross-presentation of peptides derived from exogenous Ags on MHC class I molecules is of only marginally lower efficiency ( approximately 1- to 10-fold) than the classical MHC class II pathway in vitro and in vivo. Thus, Ag quantities required for cross-presentation and cross-priming are similar to those required for fueling the MHC class II pathway. |
| spellingShingle | Storni, T Bachmann, M Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title | Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title_full | Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title_fullStr | Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title_full_unstemmed | Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title_short | Loading of MHC class I and II presentation pathways by exogenous antigens: a quantitative in vivo comparison. |
| title_sort | loading of mhc class i and ii presentation pathways by exogenous antigens a quantitative in vivo comparison |
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