Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen
Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamush...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Springer Nature
2020
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| _version_ | 1826290499335487488 |
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| author | Mika-Gospodorz, B Giengkam, S Westermann, AJ Wongsantichon, J Kion-Crosby, W Chuenklin, S Wang, LC Sunyakumthorn, P Sobota, RM Subbian, S Vogel, J Barquist, L Salje, J |
| author_facet | Mika-Gospodorz, B Giengkam, S Westermann, AJ Wongsantichon, J Kion-Crosby, W Chuenklin, S Wang, LC Sunyakumthorn, P Sobota, RM Subbian, S Vogel, J Barquist, L Salje, J |
| author_sort | Mika-Gospodorz, B |
| collection | OXFORD |
| description | Studying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot. |
| first_indexed | 2024-03-07T02:45:07Z |
| format | Journal article |
| id | oxford-uuid:abca48e9-1a18-492f-8638-f9af73eb2ffc |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:45:07Z |
| publishDate | 2020 |
| publisher | Springer Nature |
| record_format | dspace |
| spelling | oxford-uuid:abca48e9-1a18-492f-8638-f9af73eb2ffc2022-03-27T03:24:20ZDual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogenJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:abca48e9-1a18-492f-8638-f9af73eb2ffcEnglishSymplectic ElementsSpringer Nature2020Mika-Gospodorz, BGiengkam, SWestermann, AJWongsantichon, JKion-Crosby, WChuenklin, SWang, LCSunyakumthorn, PSobota, RMSubbian, SVogel, JBarquist, LSalje, JStudying emerging or neglected pathogens is often challenging due to insufficient information and absence of genetic tools. Dual RNA-seq provides insights into host-pathogen interactions, and is particularly informative for intracellular organisms. Here we apply dual RNA-seq to Orientia tsutsugamushi (Ot), an obligate intracellular bacterium that causes the vector-borne human disease scrub typhus. Half the Ot genome is composed of repetitive DNA, and there is minimal collinearity in gene order between strains. Integrating RNA-seq, comparative genomics, proteomics, and machine learning to study the transcriptional architecture of Ot, we find evidence for wide-spread post-transcriptional antisense regulation. Comparing the host response to two clinical isolates, we identify distinct immune response networks for each strain, leading to predictions of relative virulence that are validated in a mouse infection model. Thus, dual RNA-seq can provide insight into the biology and host-pathogen interactions of a poorly characterized and genetically intractable organism such as Ot. |
| spellingShingle | Mika-Gospodorz, B Giengkam, S Westermann, AJ Wongsantichon, J Kion-Crosby, W Chuenklin, S Wang, LC Sunyakumthorn, P Sobota, RM Subbian, S Vogel, J Barquist, L Salje, J Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title_full | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title_fullStr | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title_full_unstemmed | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title_short | Dual RNA-seq of Orientia tsutsugamushi informs on host-pathogen interactions for this neglected intracellular human pathogen |
| title_sort | dual rna seq of orientia tsutsugamushi informs on host pathogen interactions for this neglected intracellular human pathogen |
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