Single-molecule detection of coumarin-120
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence life...
| Main Authors: | , , |
|---|---|
| Format: | Journal article |
| Published: |
1997
|
| Summary: | Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasensitive DNA characterization. |
|---|