Single-molecule detection of coumarin-120
Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence life...
| Egile Nagusiak: | , , |
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| Formatua: | Journal article |
| Argitaratua: |
1997
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| _version_ | 1826290547987316736 |
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| author | Brand, L Eggeling, C Seidel, C |
| author_facet | Brand, L Eggeling, C Seidel, C |
| author_sort | Brand, L |
| collection | OXFORD |
| description | Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasensitive DNA characterization. |
| first_indexed | 2024-03-07T02:45:52Z |
| format | Journal article |
| id | oxford-uuid:ac0cc1fc-fcce-4fd6-a624-7b60406c14b9 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:45:52Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:ac0cc1fc-fcce-4fd6-a624-7b60406c14b92022-03-27T03:25:59ZSingle-molecule detection of coumarin-120Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ac0cc1fc-fcce-4fd6-a624-7b60406c14b9Symplectic Elements at Oxford1997Brand, LEggeling, CSeidel, CTwo-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence lifetimes. This suggests applications in current projects for ultrasensitive DNA characterization. |
| spellingShingle | Brand, L Eggeling, C Seidel, C Single-molecule detection of coumarin-120 |
| title | Single-molecule detection of coumarin-120 |
| title_full | Single-molecule detection of coumarin-120 |
| title_fullStr | Single-molecule detection of coumarin-120 |
| title_full_unstemmed | Single-molecule detection of coumarin-120 |
| title_short | Single-molecule detection of coumarin-120 |
| title_sort | single molecule detection of coumarin 120 |
| work_keys_str_mv | AT brandl singlemoleculedetectionofcoumarin120 AT eggelingc singlemoleculedetectionofcoumarin120 AT seidelc singlemoleculedetectionofcoumarin120 |