Single-molecule detection of coumarin-120

Two-photon excitation with a mode-locked titanium: sapphire laser at 700 nm and confocal fluorescence microscopy have been used to detect single Coumarin-120 (C-120) molecules. The dye C-120 is quenched by the nucleobases, if coupled to nucleotides, resuiting in nucleobase-specific fluorescence life...

Täydet tiedot

Bibliografiset tiedot
Päätekijät: Brand, L, Eggeling, C, Seidel, C
Aineistotyyppi: Journal article
Julkaistu: 1997