Isolation and culture of motor neurons from the newborn mouse spinal cord.
A protocol for the isolation and culture of motor neurons from postnatal day 1 mouse spinal cord is described. After 72 h in culture, phase contrast microscopy reveals healthy cells with motor neuronal morphology and extensive neuritic processes. These neurons express the 75-kDa low-affinity neurotr...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2004
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| _version_ | 1797088204701040640 |
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| author | Anderson, K Potter, A Piccenna, L Quah, A Davies, K Cheema, S |
| author_facet | Anderson, K Potter, A Piccenna, L Quah, A Davies, K Cheema, S |
| author_sort | Anderson, K |
| collection | OXFORD |
| description | A protocol for the isolation and culture of motor neurons from postnatal day 1 mouse spinal cord is described. After 72 h in culture, phase contrast microscopy reveals healthy cells with motor neuronal morphology and extensive neuritic processes. These neurons express the 75-kDa low-affinity neurotrophin receptor (p75NTR) and choline acetyltransferase (ChAT), both proteins are specifically expressed by neonatal and embryonic motor neurons in vivo. This protocol can be adapted for various postnatal motor neuron assays. |
| first_indexed | 2024-03-07T02:46:39Z |
| format | Journal article |
| id | oxford-uuid:ac440c53-b1ae-4ab5-9bd1-b391749da61c |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:46:39Z |
| publishDate | 2004 |
| record_format | dspace |
| spelling | oxford-uuid:ac440c53-b1ae-4ab5-9bd1-b391749da61c2022-03-27T03:27:41ZIsolation and culture of motor neurons from the newborn mouse spinal cord.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ac440c53-b1ae-4ab5-9bd1-b391749da61cEnglishSymplectic Elements at Oxford2004Anderson, KPotter, APiccenna, LQuah, ADavies, KCheema, SA protocol for the isolation and culture of motor neurons from postnatal day 1 mouse spinal cord is described. After 72 h in culture, phase contrast microscopy reveals healthy cells with motor neuronal morphology and extensive neuritic processes. These neurons express the 75-kDa low-affinity neurotrophin receptor (p75NTR) and choline acetyltransferase (ChAT), both proteins are specifically expressed by neonatal and embryonic motor neurons in vivo. This protocol can be adapted for various postnatal motor neuron assays. |
| spellingShingle | Anderson, K Potter, A Piccenna, L Quah, A Davies, K Cheema, S Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title | Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title_full | Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title_fullStr | Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title_full_unstemmed | Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title_short | Isolation and culture of motor neurons from the newborn mouse spinal cord. |
| title_sort | isolation and culture of motor neurons from the newborn mouse spinal cord |
| work_keys_str_mv | AT andersonk isolationandcultureofmotorneuronsfromthenewbornmousespinalcord AT pottera isolationandcultureofmotorneuronsfromthenewbornmousespinalcord AT piccennal isolationandcultureofmotorneuronsfromthenewbornmousespinalcord AT quaha isolationandcultureofmotorneuronsfromthenewbornmousespinalcord AT daviesk isolationandcultureofmotorneuronsfromthenewbornmousespinalcord AT cheemas isolationandcultureofmotorneuronsfromthenewbornmousespinalcord |