Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells.
Neutralizing anti-IL-2, anti-IL-3, and anti-IL-6 monoclonal antibodies (MAbs) were used for the immunoenzymatic detection of the respective cytokines in blast cells of 38 patients with acute myeloid (AML) and lymphoid (ALL) leukemias by the APAAP-technique. In 20/24 AML-cases (83%) blast cells showe...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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1992
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| _version_ | 1797088247808000000 |
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| author | Schwonzen, M Pfreundschuh, M Pohl, C Schuh, A Lathan, B Diehl, V |
| author_facet | Schwonzen, M Pfreundschuh, M Pohl, C Schuh, A Lathan, B Diehl, V |
| author_sort | Schwonzen, M |
| collection | OXFORD |
| description | Neutralizing anti-IL-2, anti-IL-3, and anti-IL-6 monoclonal antibodies (MAbs) were used for the immunoenzymatic detection of the respective cytokines in blast cells of 38 patients with acute myeloid (AML) and lymphoid (ALL) leukemias by the APAAP-technique. In 20/24 AML-cases (83%) blast cells showed intranuclear staining with MAb anti-IL-2 (DMS-1). In 17 cases reaction was restricted to the nucleoli, in 4 cases additional cytoplasmic staining was observed. Only 2/13 (15%) of the ALL cases showed anti-IL-2 staining. In contrast to IL-2, neither IL-3, IL-6 nor IL-2R alpha-chains were detected in any of the acute leukemias tested. The anti-IL-2 staining of nucleoli in AML cells is distinct from the cytoplasmic staining which is observed in PHA-activated normal lymphocytes and in a minority of AML cells. |
| first_indexed | 2024-03-07T02:47:17Z |
| format | Journal article |
| id | oxford-uuid:ac7557c5-9d3d-48e8-8dd7-f6726ab12f4a |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:47:17Z |
| publishDate | 1992 |
| record_format | dspace |
| spelling | oxford-uuid:ac7557c5-9d3d-48e8-8dd7-f6726ab12f4a2022-03-27T03:29:10ZBinding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ac7557c5-9d3d-48e8-8dd7-f6726ab12f4aEnglishSymplectic Elements at Oxford1992Schwonzen, MPfreundschuh, MPohl, CSchuh, ALathan, BDiehl, VNeutralizing anti-IL-2, anti-IL-3, and anti-IL-6 monoclonal antibodies (MAbs) were used for the immunoenzymatic detection of the respective cytokines in blast cells of 38 patients with acute myeloid (AML) and lymphoid (ALL) leukemias by the APAAP-technique. In 20/24 AML-cases (83%) blast cells showed intranuclear staining with MAb anti-IL-2 (DMS-1). In 17 cases reaction was restricted to the nucleoli, in 4 cases additional cytoplasmic staining was observed. Only 2/13 (15%) of the ALL cases showed anti-IL-2 staining. In contrast to IL-2, neither IL-3, IL-6 nor IL-2R alpha-chains were detected in any of the acute leukemias tested. The anti-IL-2 staining of nucleoli in AML cells is distinct from the cytoplasmic staining which is observed in PHA-activated normal lymphocytes and in a minority of AML cells. |
| spellingShingle | Schwonzen, M Pfreundschuh, M Pohl, C Schuh, A Lathan, B Diehl, V Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title | Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title_full | Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title_fullStr | Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title_full_unstemmed | Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title_short | Binding of monoclonal anti-interleukin 2 antibody to nucleoli in acute leukemia blast cells. |
| title_sort | binding of monoclonal anti interleukin 2 antibody to nucleoli in acute leukemia blast cells |
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