Crystal structure of an octameric RuvA-Holliday junction complex.
Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease Ru...
| Main Authors: | , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
1998
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| _version_ | 1826290744162254848 |
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| author | Roe, S Barlow, T Brown, T Oram, M Keeley, A Tsaneva, I Pearl, L |
| author_facet | Roe, S Barlow, T Brown, T Oram, M Keeley, A Tsaneva, I Pearl, L |
| author_sort | Roe, S |
| collection | OXFORD |
| description | Holliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA. |
| first_indexed | 2024-03-07T02:48:52Z |
| format | Journal article |
| id | oxford-uuid:acfd1e6f-3c0c-482b-a4e9-8432d54c28a8 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:48:52Z |
| publishDate | 1998 |
| record_format | dspace |
| spelling | oxford-uuid:acfd1e6f-3c0c-482b-a4e9-8432d54c28a82022-03-27T03:32:27ZCrystal structure of an octameric RuvA-Holliday junction complex.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:acfd1e6f-3c0c-482b-a4e9-8432d54c28a8EnglishSymplectic Elements at Oxford1998Roe, SBarlow, TBrown, TOram, MKeeley, ATsaneva, IPearl, LHolliday junctions occur as intermediates in homologous recombination and DNA repair. In bacteria, resolution of Holliday junctions is accomplished by the RuvABC system, consisting of a junction-specific helicase complex RuvAB, which promotes branch migration, and a junction-specific endonuclease RuvC, which nicks two strands. The crystal structure of a complex between the RuvA protein of M. leprae and a synthetic four-way junction has now been determined. Rather than binding on the open surface of a RuvA tetramer as previously suggested, the DNA is sandwiched between two RuvA tetramers, which form a closed octameric shell, stabilized by a conserved tetramer-tetramer interface. Interactions between the DNA backbone and helix-hairpin-helix motifs from both tetramers suggest a mechanism for strand separation promoted by RuvA. |
| spellingShingle | Roe, S Barlow, T Brown, T Oram, M Keeley, A Tsaneva, I Pearl, L Crystal structure of an octameric RuvA-Holliday junction complex. |
| title | Crystal structure of an octameric RuvA-Holliday junction complex. |
| title_full | Crystal structure of an octameric RuvA-Holliday junction complex. |
| title_fullStr | Crystal structure of an octameric RuvA-Holliday junction complex. |
| title_full_unstemmed | Crystal structure of an octameric RuvA-Holliday junction complex. |
| title_short | Crystal structure of an octameric RuvA-Holliday junction complex. |
| title_sort | crystal structure of an octameric ruva holliday junction complex |
| work_keys_str_mv | AT roes crystalstructureofanoctamericruvahollidayjunctioncomplex AT barlowt crystalstructureofanoctamericruvahollidayjunctioncomplex AT brownt crystalstructureofanoctamericruvahollidayjunctioncomplex AT oramm crystalstructureofanoctamericruvahollidayjunctioncomplex AT keeleya crystalstructureofanoctamericruvahollidayjunctioncomplex AT tsanevai crystalstructureofanoctamericruvahollidayjunctioncomplex AT pearll crystalstructureofanoctamericruvahollidayjunctioncomplex |