KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development
Fertilization is a key event for sexually reproducing plants. Pollen–stigma adhesion, which is the first step in male–female interaction during fertilization, requires proper pollen wall patterning. Callose, which is a β-1.3-glucan, is an essential polysaccharide that is required for pollen developm...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Journal article |
| Language: | English |
| Published: |
MDPI
2022
|
| _version_ | 1797111837031923712 |
|---|---|
| author | Kanaoka, MM Shimizu, KK Xie, B Urban, S Freeman, M Hong, Z Okada, K |
| author_facet | Kanaoka, MM Shimizu, KK Xie, B Urban, S Freeman, M Hong, Z Okada, K |
| author_sort | Kanaoka, MM |
| collection | OXFORD |
| description | Fertilization is a key event for sexually reproducing plants. Pollen–stigma adhesion, which is the first step in male–female interaction during fertilization, requires proper pollen wall patterning. Callose, which is a β-1.3-glucan, is an essential polysaccharide that is required for pollen development and pollen wall formation. Mutations in <i>CALLOSE SYNTHASE 5 (CalS5)</i> disrupt male meiotic callose accumulation; however, how CalS5 activity and callose synthesis are regulated is not fully understood. In this paper, we report the isolation of a <i>kompeito-1 (kom-1)</i> mutant defective in pollen wall patterning and pollen–stigma adhesion in <i>Arabidopsis thaliana</i>. Callose was not accumulated in <i>kom-1</i> meiocytes or microspores, which was very similar to the <i>cals5</i> mutant. The <i>KOM</i> gene encoded a member of a subclass of Rhomboid serine protease proteins that lacked active site residues. KOM was localized to the Golgi apparatus, and both <i>KOM</i> and <i>CalS5</i> genes were highly expressed in meiocytes. A 220 kDa CalS5 protein was detected in wild-type (Col-0) floral buds but was dramatically reduced in <i>kom-1</i>. These results suggested that KOM was required for CalS5 protein accumulation, leading to the regulation of meiocyte-specific callose accumulation and pollen wall formation. |
| first_indexed | 2024-03-07T08:16:01Z |
| format | Journal article |
| id | oxford-uuid:ad0eb89d-76d4-4dce-9319-17465902f720 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:16:01Z |
| publishDate | 2022 |
| publisher | MDPI |
| record_format | dspace |
| spelling | oxford-uuid:ad0eb89d-76d4-4dce-9319-17465902f7202023-12-21T13:50:09ZKOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall developmentJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ad0eb89d-76d4-4dce-9319-17465902f720EnglishSymplectic ElementsMDPI2022Kanaoka, MMShimizu, KKXie, BUrban, SFreeman, MHong, ZOkada, KFertilization is a key event for sexually reproducing plants. Pollen–stigma adhesion, which is the first step in male–female interaction during fertilization, requires proper pollen wall patterning. Callose, which is a β-1.3-glucan, is an essential polysaccharide that is required for pollen development and pollen wall formation. Mutations in <i>CALLOSE SYNTHASE 5 (CalS5)</i> disrupt male meiotic callose accumulation; however, how CalS5 activity and callose synthesis are regulated is not fully understood. In this paper, we report the isolation of a <i>kompeito-1 (kom-1)</i> mutant defective in pollen wall patterning and pollen–stigma adhesion in <i>Arabidopsis thaliana</i>. Callose was not accumulated in <i>kom-1</i> meiocytes or microspores, which was very similar to the <i>cals5</i> mutant. The <i>KOM</i> gene encoded a member of a subclass of Rhomboid serine protease proteins that lacked active site residues. KOM was localized to the Golgi apparatus, and both <i>KOM</i> and <i>CalS5</i> genes were highly expressed in meiocytes. A 220 kDa CalS5 protein was detected in wild-type (Col-0) floral buds but was dramatically reduced in <i>kom-1</i>. These results suggested that KOM was required for CalS5 protein accumulation, leading to the regulation of meiocyte-specific callose accumulation and pollen wall formation. |
| spellingShingle | Kanaoka, MM Shimizu, KK Xie, B Urban, S Freeman, M Hong, Z Okada, K KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title | KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title_full | KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title_fullStr | KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title_full_unstemmed | KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title_short | KOMPEITO, an atypical Arabidopsis Rhomboid-related gene, is required for callose accumulation and pollen wall development |
| title_sort | kompeito an atypical arabidopsis rhomboid related gene is required for callose accumulation and pollen wall development |
| work_keys_str_mv | AT kanaokamm kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT shimizukk kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT xieb kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT urbans kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT freemanm kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT hongz kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment AT okadak kompeitoanatypicalarabidopsisrhomboidrelatedgeneisrequiredforcalloseaccumulationandpollenwalldevelopment |