The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis
In the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell m...
| Main Authors: | , , , , |
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| Format: | Conference item |
| Language: | English |
| Published: |
Chemical and Biological Microsystems Society
2011
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| Summary: | In the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell membrane deformation, and simultaneous quantification of both intracellular calcium concentration ([Ca2+]i) and transmembrane transfer of extracellular membrane-impermeable probes. We have highlighted novel aspects of microbubble-cluster dynamics combined with localised cell membrane strain, which could be responsible for membrane permeabilisation and transmembrane pore formation correlated with the transduction of intracellular biochemical signals (i.e. [Ca2+]i influx) as a result of microbubble-cell interaction. |
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