The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis
In the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell m...
| Main Authors: | , , , , |
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| Format: | Conference item |
| Language: | English |
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Chemical and Biological Microsystems Society
2011
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| _version_ | 1826311039171428352 |
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| author | Bose, N Carugo, D Maiti, TK Zhang, X Chakraborty, S |
| author_facet | Bose, N Carugo, D Maiti, TK Zhang, X Chakraborty, S |
| author_sort | Bose, N |
| collection | OXFORD |
| description | In the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell membrane deformation, and simultaneous quantification of both intracellular calcium concentration ([Ca2+]i) and transmembrane transfer of extracellular membrane-impermeable probes. We have highlighted novel aspects of microbubble-cluster dynamics combined with localised cell membrane strain, which could be responsible for membrane permeabilisation and transmembrane pore formation correlated with the transduction of intracellular biochemical signals (i.e. [Ca2+]i influx) as a result of microbubble-cell interaction. |
| first_indexed | 2024-03-07T08:02:28Z |
| format | Conference item |
| id | oxford-uuid:ad12886c-310e-46cd-9de5-216fb057976b |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:02:28Z |
| publishDate | 2011 |
| publisher | Chemical and Biological Microsystems Society |
| record_format | dspace |
| spelling | oxford-uuid:ad12886c-310e-46cd-9de5-216fb057976b2023-10-09T17:20:23ZThe role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysisConference itemhttp://purl.org/coar/resource_type/c_5794uuid:ad12886c-310e-46cd-9de5-216fb057976bEnglishSymplectic Elements Chemical and Biological Microsystems Society2011Bose, NCarugo, DMaiti, TKZhang, XChakraborty, SIn the present study we have investigated the sonoporation dynamics in a single cell using a novel microfluidic-based approach. Our methodology has successfully addressed the biophysical mechanisms underlying US-induced cell membrane sonoporation by performing in situ measurement of localised cell membrane deformation, and simultaneous quantification of both intracellular calcium concentration ([Ca2+]i) and transmembrane transfer of extracellular membrane-impermeable probes. We have highlighted novel aspects of microbubble-cluster dynamics combined with localised cell membrane strain, which could be responsible for membrane permeabilisation and transmembrane pore formation correlated with the transduction of intracellular biochemical signals (i.e. [Ca2+]i influx) as a result of microbubble-cell interaction. |
| spellingShingle | Bose, N Carugo, D Maiti, TK Zhang, X Chakraborty, S The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title | The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title_full | The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title_fullStr | The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title_full_unstemmed | The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title_short | The role of cell membrane strain in sonoporation characterised by microfluidic-based single-cell analysis |
| title_sort | role of cell membrane strain in sonoporation characterised by microfluidic based single cell analysis |
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