Quantitation of ER structure and function
The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a...
| Main Authors: | , , , , , |
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| Other Authors: | |
| Format: | Book section |
| Language: | English |
| Published: |
Humana Press
2017
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| _version_ | 1797111410957746176 |
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| author | Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ |
| author2 | Hawes, C |
| author_facet | Hawes, C Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ |
| author_sort | Fricker, M |
| collection | OXFORD |
| description | The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available. |
| first_indexed | 2024-03-07T08:09:57Z |
| format | Book section |
| id | oxford-uuid:ad194a39-1aab-45d2-8402-b3b7aed848bf |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T08:09:57Z |
| publishDate | 2017 |
| publisher | Humana Press |
| record_format | dspace |
| spelling | oxford-uuid:ad194a39-1aab-45d2-8402-b3b7aed848bf2023-11-17T09:42:30ZQuantitation of ER structure and functionBook sectionhttp://purl.org/coar/resource_type/c_1843uuid:ad194a39-1aab-45d2-8402-b3b7aed848bfEnglishSymplectic Elements at OxfordHumana Press2017Fricker, MHeaton, LJones, NObara, BMüller, SJMeyer, AJHawes, CKriechbaumer, VThe plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available. |
| spellingShingle | Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ Quantitation of ER structure and function |
| title | Quantitation of ER structure and function |
| title_full | Quantitation of ER structure and function |
| title_fullStr | Quantitation of ER structure and function |
| title_full_unstemmed | Quantitation of ER structure and function |
| title_short | Quantitation of ER structure and function |
| title_sort | quantitation of er structure and function |
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