Quantitation of ER structure and function
The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a...
Main Authors: | , , , , , |
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Other Authors: | |
Format: | Book section |
Language: | English |
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Humana Press
2017
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_version_ | 1797111410957746176 |
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author | Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ |
author2 | Hawes, C |
author_facet | Hawes, C Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ |
author_sort | Fricker, M |
collection | OXFORD |
description | The plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available. |
first_indexed | 2024-03-07T08:09:57Z |
format | Book section |
id | oxford-uuid:ad194a39-1aab-45d2-8402-b3b7aed848bf |
institution | University of Oxford |
language | English |
last_indexed | 2024-03-07T08:09:57Z |
publishDate | 2017 |
publisher | Humana Press |
record_format | dspace |
spelling | oxford-uuid:ad194a39-1aab-45d2-8402-b3b7aed848bf2023-11-17T09:42:30ZQuantitation of ER structure and functionBook sectionhttp://purl.org/coar/resource_type/c_1843uuid:ad194a39-1aab-45d2-8402-b3b7aed848bfEnglishSymplectic Elements at OxfordHumana Press2017Fricker, MHeaton, LJones, NObara, BMüller, SJMeyer, AJHawes, CKriechbaumer, VThe plant endoplasmic reticulum forms a network of tubules connected by three-way junctions or sheet-like cisternae. Although the network is three-dimensional, in many plant cells, it is constrained to a thin volume sandwiched between the vacuole and plasma membrane, effectively restricting it to a 2-D planar network. The structure of the network, and the morphology of the tubules and cisternae can be automatically extracted following intensity-independent edge-enhancement and various segmentation techniques to give an initial pixel-based skeleton, which is then converted to a graph representation. Collectively, this approach yields a wealth of quantitative metrics for ER structure and can be used to describe the effects of pharmacological treatments or genetic manipulation. The software is publicly available. |
spellingShingle | Fricker, M Heaton, L Jones, N Obara, B Müller, SJ Meyer, AJ Quantitation of ER structure and function |
title | Quantitation of ER structure and function |
title_full | Quantitation of ER structure and function |
title_fullStr | Quantitation of ER structure and function |
title_full_unstemmed | Quantitation of ER structure and function |
title_short | Quantitation of ER structure and function |
title_sort | quantitation of er structure and function |
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