Structural basis of vesicle formation at the inner nuclear membrane
Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understa...
| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
Cell Press
2015
|
| _version_ | 1826290780514287616 |
|---|---|
| author | Hagen, C Dent, K Zeev-Ben-Mordehai, Z Grange, M Bosse, J Whittle, C Klupp, B Siebert, C Vasishtan, D Bäuerlein, F Cheleski, J Werner, S Guttmann, P Rehbein, S Henzler, K Demmerle, J Adler, B Koszinowski, U Schermelleh, L Schneider, G Enquist, L Plitzko, J Mettenleiter, T Grünewald, K |
| author_facet | Hagen, C Dent, K Zeev-Ben-Mordehai, Z Grange, M Bosse, J Whittle, C Klupp, B Siebert, C Vasishtan, D Bäuerlein, F Cheleski, J Werner, S Guttmann, P Rehbein, S Henzler, K Demmerle, J Adler, B Koszinowski, U Schermelleh, L Schneider, G Enquist, L Plitzko, J Mettenleiter, T Grünewald, K |
| author_sort | Hagen, C |
| collection | OXFORD |
| description | Vesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress complex (NEC). Using a multi-modal imaging approach, we visualized the NEC in situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexagonal lattices at its membrane-proximal and membrane-distant faces, respectively. NEC coat architecture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM. |
| first_indexed | 2024-03-07T02:49:25Z |
| format | Journal article |
| id | oxford-uuid:ad28083d-3351-476e-8f50-432c1b3e7598 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:49:25Z |
| publishDate | 2015 |
| publisher | Cell Press |
| record_format | dspace |
| spelling | oxford-uuid:ad28083d-3351-476e-8f50-432c1b3e75982022-03-27T03:33:46ZStructural basis of vesicle formation at the inner nuclear membraneJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ad28083d-3351-476e-8f50-432c1b3e7598EnglishSymplectic Elements at OxfordCell Press2015Hagen, CDent, KZeev-Ben-Mordehai, ZGrange, MBosse, JWhittle, CKlupp, BSiebert, CVasishtan, DBäuerlein, FCheleski, JWerner, SGuttmann, PRehbein, SHenzler, KDemmerle, JAdler, BKoszinowski, USchermelleh, LSchneider, GEnquist, LPlitzko, JMettenleiter, TGrünewald, KVesicular nucleo-cytoplasmic transport is becoming recognized as a general cellular mechanism for translocation of large cargoes across the nuclear envelope. Cargo is recruited, enveloped at the inner nuclear membrane (INM), and delivered by membrane fusion at the outer nuclear membrane. To understand the structural underpinning for this trafficking, we investigated nuclear egress of progeny herpesvirus capsids where capsid envelopment is mediated by two viral proteins, forming the nuclear egress complex (NEC). Using a multi-modal imaging approach, we visualized the NEC in situ forming coated vesicles of defined size. Cellular electron cryo-tomography revealed a protein layer showing two distinct hexagonal lattices at its membrane-proximal and membrane-distant faces, respectively. NEC coat architecture was determined by combining this information with integrative modeling using small-angle X-ray scattering data. The molecular arrangement of the NEC establishes the basic mechanism for budding and scission of tailored vesicles at the INM. |
| spellingShingle | Hagen, C Dent, K Zeev-Ben-Mordehai, Z Grange, M Bosse, J Whittle, C Klupp, B Siebert, C Vasishtan, D Bäuerlein, F Cheleski, J Werner, S Guttmann, P Rehbein, S Henzler, K Demmerle, J Adler, B Koszinowski, U Schermelleh, L Schneider, G Enquist, L Plitzko, J Mettenleiter, T Grünewald, K Structural basis of vesicle formation at the inner nuclear membrane |
| title | Structural basis of vesicle formation at the inner nuclear membrane |
| title_full | Structural basis of vesicle formation at the inner nuclear membrane |
| title_fullStr | Structural basis of vesicle formation at the inner nuclear membrane |
| title_full_unstemmed | Structural basis of vesicle formation at the inner nuclear membrane |
| title_short | Structural basis of vesicle formation at the inner nuclear membrane |
| title_sort | structural basis of vesicle formation at the inner nuclear membrane |
| work_keys_str_mv | AT hagenc structuralbasisofvesicleformationattheinnernuclearmembrane AT dentk structuralbasisofvesicleformationattheinnernuclearmembrane AT zeevbenmordehaiz structuralbasisofvesicleformationattheinnernuclearmembrane AT grangem structuralbasisofvesicleformationattheinnernuclearmembrane AT bossej structuralbasisofvesicleformationattheinnernuclearmembrane AT whittlec structuralbasisofvesicleformationattheinnernuclearmembrane AT kluppb structuralbasisofvesicleformationattheinnernuclearmembrane AT siebertc structuralbasisofvesicleformationattheinnernuclearmembrane AT vasishtand structuralbasisofvesicleformationattheinnernuclearmembrane AT bauerleinf structuralbasisofvesicleformationattheinnernuclearmembrane AT cheleskij structuralbasisofvesicleformationattheinnernuclearmembrane AT werners structuralbasisofvesicleformationattheinnernuclearmembrane AT guttmannp structuralbasisofvesicleformationattheinnernuclearmembrane AT rehbeins structuralbasisofvesicleformationattheinnernuclearmembrane AT henzlerk structuralbasisofvesicleformationattheinnernuclearmembrane AT demmerlej structuralbasisofvesicleformationattheinnernuclearmembrane AT adlerb structuralbasisofvesicleformationattheinnernuclearmembrane AT koszinowskiu structuralbasisofvesicleformationattheinnernuclearmembrane AT schermellehl structuralbasisofvesicleformationattheinnernuclearmembrane AT schneiderg structuralbasisofvesicleformationattheinnernuclearmembrane AT enquistl structuralbasisofvesicleformationattheinnernuclearmembrane AT plitzkoj structuralbasisofvesicleformationattheinnernuclearmembrane AT mettenleitert structuralbasisofvesicleformationattheinnernuclearmembrane AT grunewaldk structuralbasisofvesicleformationattheinnernuclearmembrane |