Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway
Layered double hydroxides (LDHs) are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA bioconjugates via a microscopic approach and analyzed the internalization pathway by dissipative p...
| Main Authors: | , , , , , , , , |
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| 格式: | Journal article |
| 出版: |
Elsevier
2017
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| _version_ | 1826290820343398400 |
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| author | Li, Y Bao, W Wu, H Wang, J Zhang, Y Wan, Y Cao, D O’Hare, D Wang, Q |
| author_facet | Li, Y Bao, W Wu, H Wang, J Zhang, Y Wan, Y Cao, D O’Hare, D Wang, Q |
| author_sort | Li, Y |
| collection | OXFORD |
| description | Layered double hydroxides (LDHs) are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA bioconjugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics (DPD) simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction analysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex, while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simulations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane (PM), while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems. |
| first_indexed | 2024-03-07T02:50:03Z |
| format | Journal article |
| id | oxford-uuid:ad5fbc42-bf9a-4467-bf3b-a66e505cb0c1 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:50:03Z |
| publishDate | 2017 |
| publisher | Elsevier |
| record_format | dspace |
| spelling | oxford-uuid:ad5fbc42-bf9a-4467-bf3b-a66e505cb0c12022-03-27T03:35:10ZDelaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathwayJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ad5fbc42-bf9a-4467-bf3b-a66e505cb0c1Symplectic Elements at OxfordElsevier2017Li, YBao, WWu, HWang, JZhang, YWan, YCao, DO’Hare, DWang, QLayered double hydroxides (LDHs) are effective molecular carriers in cytological research, gene therapy, and transgenic applications. Herein, we investigated the internalization behavior of the LDH-DNA bioconjugates via a microscopic approach and analyzed the internalization pathway by dissipative particle dynamics (DPD) simulations. We experimentally found that LDH can efficiently carry DNA into the nucleus of cell in BY-2 suspension cells. Furthermore, atomic force microscopy and X-ray diffraction analysis demonstrated that the LDH-DNA bioconjugates mainly exist as a DNA-LDH-DNA sandwich complex, while the LDH-DNA-LDH sandwich complex and DNA-LDH complex cannot be excluded. The DPD simulations further indicated that only the DNA-LDH-DNA sandwich structure could penetrate the plasma membrane (PM), while PM is impermeable to the LDH-DNA-LDH sandwich complex and the DNA-LDH complex. This work provides novel perspective for understanding the membrane penetration mechanism of LDH nano-sheets and new insights into the design of novel molecular delivery systems. |
| spellingShingle | Li, Y Bao, W Wu, H Wang, J Zhang, Y Wan, Y Cao, D O’Hare, D Wang, Q Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title_full | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title_fullStr | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title_full_unstemmed | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title_short | Delaminated layered double hydroxide delivers DNA molecules as sandwich nanostructure into cells via a non-endocytic pathway |
| title_sort | delaminated layered double hydroxide delivers dna molecules as sandwich nanostructure into cells via a non endocytic pathway |
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