The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi.
Mycelial fungi have a growth form which is unique among multicellular organisms. The data presented here suggest that they have developed a unique solution to internal solute translocation involving a complex, extended vacuole. In all filamentous fungi examined, this extended vacuole forms an interc...
| Principais autores: | , , , , |
|---|---|
| Formato: | Journal article |
| Idioma: | English |
| Publicado em: |
2006
|
| _version_ | 1826290836598423552 |
|---|---|
| author | Darrah, P Tlalka, M Ashford, A Watkinson, S Fricker, M |
| author_facet | Darrah, P Tlalka, M Ashford, A Watkinson, S Fricker, M |
| author_sort | Darrah, P |
| collection | OXFORD |
| description | Mycelial fungi have a growth form which is unique among multicellular organisms. The data presented here suggest that they have developed a unique solution to internal solute translocation involving a complex, extended vacuole. In all filamentous fungi examined, this extended vacuole forms an interconnected network, dynamically linked by tubules, which has been hypothesized to act as an internal distribution system. We have tested this hypothesis directly by quantifying solute movement within the organelle by photobleaching a fluorescent vacuolar marker. Predictive simulation models were then used to determine the transport characteristics over extended length scales. This modeling showed that the vacuolar organelle forms a functionally important, bidirectional diffusive transport pathway over distances of millimeters to centimeters. Flux through the pathway is regulated by the dynamic tubular connections involving homotypic fusion and fission. There is also a strongly predicted interaction among vacuolar organization, predicted diffusion transport distances, and the architecture of the branching colony margin. |
| first_indexed | 2024-03-07T02:50:18Z |
| format | Journal article |
| id | oxford-uuid:ad7539a6-ffdf-4805-907a-3c3c07946f85 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:50:18Z |
| publishDate | 2006 |
| record_format | dspace |
| spelling | oxford-uuid:ad7539a6-ffdf-4805-907a-3c3c07946f852022-03-27T03:35:43ZThe vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ad7539a6-ffdf-4805-907a-3c3c07946f85EnglishSymplectic Elements at Oxford2006Darrah, PTlalka, MAshford, AWatkinson, SFricker, MMycelial fungi have a growth form which is unique among multicellular organisms. The data presented here suggest that they have developed a unique solution to internal solute translocation involving a complex, extended vacuole. In all filamentous fungi examined, this extended vacuole forms an interconnected network, dynamically linked by tubules, which has been hypothesized to act as an internal distribution system. We have tested this hypothesis directly by quantifying solute movement within the organelle by photobleaching a fluorescent vacuolar marker. Predictive simulation models were then used to determine the transport characteristics over extended length scales. This modeling showed that the vacuolar organelle forms a functionally important, bidirectional diffusive transport pathway over distances of millimeters to centimeters. Flux through the pathway is regulated by the dynamic tubular connections involving homotypic fusion and fission. There is also a strongly predicted interaction among vacuolar organization, predicted diffusion transport distances, and the architecture of the branching colony margin. |
| spellingShingle | Darrah, P Tlalka, M Ashford, A Watkinson, S Fricker, M The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title | The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title_full | The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title_fullStr | The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title_full_unstemmed | The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title_short | The vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi. |
| title_sort | vacuole system is a significant intracellular pathway for longitudinal solute transport in basidiomycete fungi |
| work_keys_str_mv | AT darrahp thevacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT tlalkam thevacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT ashforda thevacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT watkinsons thevacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT frickerm thevacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT darrahp vacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT tlalkam vacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT ashforda vacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT watkinsons vacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi AT frickerm vacuolesystemisasignificantintracellularpathwayforlongitudinalsolutetransportinbasidiomycetefungi |