Reactive oxygen species produced by NADPH oxidase regulate plant cell growth.
Cell expansion is a central process in plant morphogenesis, and the elongation of roots and root hairs is essential for uptake of minerals and water from the soil. Ca2+ influx from the extracellular store is required for (and sets the rates of) cell elongation in roots. Arabidopsis thaliana rhd2 mut...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
2003
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| _version_ | 1826290878422974464 |
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| author | Foreman, J Demidchik, V Bothwell, J Mylona, P Miedema, H Torres, M Linstead, P Costa, S Brownlee, C Jones, J Davies, J Dolan, L |
| author_facet | Foreman, J Demidchik, V Bothwell, J Mylona, P Miedema, H Torres, M Linstead, P Costa, S Brownlee, C Jones, J Davies, J Dolan, L |
| author_sort | Foreman, J |
| collection | OXFORD |
| description | Cell expansion is a central process in plant morphogenesis, and the elongation of roots and root hairs is essential for uptake of minerals and water from the soil. Ca2+ influx from the extracellular store is required for (and sets the rates of) cell elongation in roots. Arabidopsis thaliana rhd2 mutants are defective in Ca2+ uptake and consequently cell expansion is compromised--rhd2 mutants have short root hairs and stunted roots. To determine the regulation of Ca2+ acquisition in growing root cells we show here that RHD2 is an NADPH oxidase, a protein that transfers electrons from NADPH to an electron acceptor leading to the formation of reactive oxygen species (ROS). We show that ROS accumulate in growing wild-type (WT) root hairs but their levels are markedly decreased in rhd2 mutants. Blocking the activity of the NADPH oxidase with diphenylene iodonium (DPI) inhibits ROS formation and phenocopies Rhd2-. Treatment of rhd2 roots with ROS partly suppresses the mutant phenotype and stimulates the activity of plasma membrane hyperpolarization-activated Ca2+ channels, the predominant root Ca2+ acquisition system. This indicates that NADPH oxidases control development by making ROS that regulate plant cell expansion through the activation of Ca2+ channels. |
| first_indexed | 2024-03-07T02:50:56Z |
| format | Journal article |
| id | oxford-uuid:ada82281-edaf-46d0-8508-b6c5187ebb00 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:50:56Z |
| publishDate | 2003 |
| record_format | dspace |
| spelling | oxford-uuid:ada82281-edaf-46d0-8508-b6c5187ebb002022-03-27T03:37:10ZReactive oxygen species produced by NADPH oxidase regulate plant cell growth.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:ada82281-edaf-46d0-8508-b6c5187ebb00EnglishSymplectic Elements at Oxford2003Foreman, JDemidchik, VBothwell, JMylona, PMiedema, HTorres, MLinstead, PCosta, SBrownlee, CJones, JDavies, JDolan, LCell expansion is a central process in plant morphogenesis, and the elongation of roots and root hairs is essential for uptake of minerals and water from the soil. Ca2+ influx from the extracellular store is required for (and sets the rates of) cell elongation in roots. Arabidopsis thaliana rhd2 mutants are defective in Ca2+ uptake and consequently cell expansion is compromised--rhd2 mutants have short root hairs and stunted roots. To determine the regulation of Ca2+ acquisition in growing root cells we show here that RHD2 is an NADPH oxidase, a protein that transfers electrons from NADPH to an electron acceptor leading to the formation of reactive oxygen species (ROS). We show that ROS accumulate in growing wild-type (WT) root hairs but their levels are markedly decreased in rhd2 mutants. Blocking the activity of the NADPH oxidase with diphenylene iodonium (DPI) inhibits ROS formation and phenocopies Rhd2-. Treatment of rhd2 roots with ROS partly suppresses the mutant phenotype and stimulates the activity of plasma membrane hyperpolarization-activated Ca2+ channels, the predominant root Ca2+ acquisition system. This indicates that NADPH oxidases control development by making ROS that regulate plant cell expansion through the activation of Ca2+ channels. |
| spellingShingle | Foreman, J Demidchik, V Bothwell, J Mylona, P Miedema, H Torres, M Linstead, P Costa, S Brownlee, C Jones, J Davies, J Dolan, L Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title | Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title_full | Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title_fullStr | Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title_full_unstemmed | Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title_short | Reactive oxygen species produced by NADPH oxidase regulate plant cell growth. |
| title_sort | reactive oxygen species produced by nadph oxidase regulate plant cell growth |
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