Identification of syntaxin 1A as a novel binding protein for presenilin-1.

Identification of syntaxin 1A as a novel binding protein for presenilin-1.

Mutations in the presenilin 1 gene have been shown to result in Alzheimer's disease. Presenilin 1 is a multi-transmembrane protein with a large hydrophilic loop near the C-terminus. This region is required for known functions of presenilin 1. We have constrained this loop within the active site...

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Մատենագիտական մանրամասներ
Հիմնական հեղինակներ: Smith, S, Anderson, H, Yu, G, Robertson, A, Allen, S, Tyler, S, Naylor, R, Mason, G, Wilcock, G, Roche, P, Fraser, P, Dawbarn, D
Ձևաչափ: Journal article
Լեզու:English
Հրապարակվել է: 2000
Նկարագրություն
Ամփոփում:Mutations in the presenilin 1 gene have been shown to result in Alzheimer's disease. Presenilin 1 is a multi-transmembrane protein with a large hydrophilic loop near the C-terminus. This region is required for known functions of presenilin 1. We have constrained this loop within the active site of the bacterial protein, thioredoxin, to mimic its native conformational state. This hybrid protein was used as bait in a yeast two hybrid screen in an attempt to identify presenilin binding proteins. By this method syntaxin 1A, a synaptic plasma membrane protein, was identified as a novel binding protein for presenilin 1. In vitro experiments confirm the two-hybrid results suggesting that PS1 binds syntaxin under physiological conditions.

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