Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines.
Locomotion of colorectal-carcinoma cells was tested in order to establish whether it might be affected by carcino-embryonic antigen (CEA). CEA production, cell growth and DNA ploidy were measured in 22 colorectal-carcinoma cell lines. A cell-invasion assay was adapted using a transfilter chamber, th...
| Main Authors: | , , , , , |
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| Format: | Journal article |
| Language: | English |
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1999
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| _version_ | 1797088779076370432 |
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| author | Kim, J Koo, K Kim, B Park, K Bicknell, D Bodmer, W |
| author_facet | Kim, J Koo, K Kim, B Park, K Bicknell, D Bodmer, W |
| author_sort | Kim, J |
| collection | OXFORD |
| description | Locomotion of colorectal-carcinoma cells was tested in order to establish whether it might be affected by carcino-embryonic antigen (CEA). CEA production, cell growth and DNA ploidy were measured in 22 colorectal-carcinoma cell lines. A cell-invasion assay was adapted using a transfilter chamber, the lower surface of which was coated with various substrates in the amount of 5 microgram/filter (CEA, type-IV collagen, laminin). Cells infiltrated into the lower surface of the filter were counted over 9-microscope fields (x400). All cell lines produced CEA, 9 producing more than 100 ng/ml medium. Of the total, 8 cell lines were diploid and 14 were aneuploid. Invasiveness, measured by the number of infiltrated cells, was highest in CEA-coated filters, and next highest in type-IV-collagen- and laminin-coated filters, in descending order (p < 0.001-0.05). Invasiveness of each cell line was closely correlated with 2 substrates. Poorly differentiated or advanced-stage tumors were more invasive than well-differentiated or early-stage tumors (p < 0.001-0. 05). However, invasiveness was not associated with DNA ploidy or CEA production. CEA may function as a chemo-attractant as well as an adhesion molecule in colorectal-carcinoma cell lines. In addition, adhesion to CEA appears to be related to type-IV collagen and laminin. |
| first_indexed | 2024-03-07T02:54:56Z |
| format | Journal article |
| id | oxford-uuid:aef2333d-d979-4156-87eb-78a7f234244d |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:54:56Z |
| publishDate | 1999 |
| record_format | dspace |
| spelling | oxford-uuid:aef2333d-d979-4156-87eb-78a7f234244d2022-03-27T03:46:13ZCarcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:aef2333d-d979-4156-87eb-78a7f234244dEnglishSymplectic Elements at Oxford1999Kim, JKoo, KKim, BPark, KBicknell, DBodmer, WLocomotion of colorectal-carcinoma cells was tested in order to establish whether it might be affected by carcino-embryonic antigen (CEA). CEA production, cell growth and DNA ploidy were measured in 22 colorectal-carcinoma cell lines. A cell-invasion assay was adapted using a transfilter chamber, the lower surface of which was coated with various substrates in the amount of 5 microgram/filter (CEA, type-IV collagen, laminin). Cells infiltrated into the lower surface of the filter were counted over 9-microscope fields (x400). All cell lines produced CEA, 9 producing more than 100 ng/ml medium. Of the total, 8 cell lines were diploid and 14 were aneuploid. Invasiveness, measured by the number of infiltrated cells, was highest in CEA-coated filters, and next highest in type-IV-collagen- and laminin-coated filters, in descending order (p < 0.001-0.05). Invasiveness of each cell line was closely correlated with 2 substrates. Poorly differentiated or advanced-stage tumors were more invasive than well-differentiated or early-stage tumors (p < 0.001-0. 05). However, invasiveness was not associated with DNA ploidy or CEA production. CEA may function as a chemo-attractant as well as an adhesion molecule in colorectal-carcinoma cell lines. In addition, adhesion to CEA appears to be related to type-IV collagen and laminin. |
| spellingShingle | Kim, J Koo, K Kim, B Park, K Bicknell, D Bodmer, W Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title | Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title_full | Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title_fullStr | Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title_full_unstemmed | Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title_short | Carcino-embryonic antigen may function as a chemo-attractant in colorectal-carcinoma cell lines. |
| title_sort | carcino embryonic antigen may function as a chemo attractant in colorectal carcinoma cell lines |
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