Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries
DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined...
| Main Authors: | , , , , , , , , |
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| Format: | Journal article |
| Language: | English |
| Published: |
Royal Society of Chemistry
2017
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| _version_ | 1826291162788397056 |
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| author | Matysiak, S Hellmuth, K El-Sagheer, A Shivalingam, A Ariyurek, Y de Jong, M Hollestelle, M Out, R Brown, T |
| author_facet | Matysiak, S Hellmuth, K El-Sagheer, A Shivalingam, A Ariyurek, Y de Jong, M Hollestelle, M Out, R Brown, T |
| author_sort | Matysiak, S |
| collection | OXFORD |
| description | DNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design. |
| first_indexed | 2024-03-07T02:55:16Z |
| format | Journal article |
| id | oxford-uuid:af0f8ccb-bdf0-406b-a1f4-d6d8507092f6 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:55:16Z |
| publishDate | 2017 |
| publisher | Royal Society of Chemistry |
| record_format | dspace |
| spelling | oxford-uuid:af0f8ccb-bdf0-406b-a1f4-d6d8507092f62022-03-27T03:47:02ZSearching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand librariesJournal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:af0f8ccb-bdf0-406b-a1f4-d6d8507092f6EnglishSymplectic Elements at OxfordRoyal Society of Chemistry2017Matysiak, SHellmuth, KEl-Sagheer, AShivalingam, AAriyurek, Yde Jong, MHollestelle, MOut, RBrown, TDNA encoded ligands are self-assembled into bivalent complexes and chemically ligated to link their identities. To demonstrate their potential as a combinatorial screening platform for avidity interactions, the optimal bivalent aptamer design (examplar ligands) for human alpha-thrombin is determined in a single round of selection and the DNA scaffold replaced with minimal impact on the final design. |
| spellingShingle | Matysiak, S Hellmuth, K El-Sagheer, A Shivalingam, A Ariyurek, Y de Jong, M Hollestelle, M Out, R Brown, T Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title | Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title_full | Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title_fullStr | Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title_full_unstemmed | Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title_short | Searching for avidity by chemical ligation of combinatorially self-assembled DNA-encoded ligand libraries |
| title_sort | searching for avidity by chemical ligation of combinatorially self assembled dna encoded ligand libraries |
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