Automatic analysis of agarose gel images
Motivation: Automatic tools to speed up routine biological processes are very much sought after in bio-medical research. Much repetitive work in molecular biology, such as allele calling in genetic analysis, can be made semi-automatic or task specific automatic by using existing techniques from comp...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Conference item |
| Published: |
2001
|
| _version_ | 1797088807548354560 |
|---|---|
| author | Adiga, P Bhomra, A Turri, MG Nicod, A Datta, SR Jeavons, P Mott, R Flint, J |
| author_facet | Adiga, P Bhomra, A Turri, MG Nicod, A Datta, SR Jeavons, P Mott, R Flint, J |
| author_sort | Adiga, P |
| collection | OXFORD |
| description | Motivation: Automatic tools to speed up routine biological processes are very much sought after in bio-medical research. Much repetitive work in molecular biology, such as allele calling in genetic analysis, can be made semi-automatic or task specific automatic by using existing techniques from computer science and signal processing. Computerized analysis is reproducible and avoids various forms of human error. Semi-automatic techniques with an interactive check on the results speed up the analysis and reduce the error. Results: We have successfully implemented an image processing software package to automatically analyze agarose gel images of polymorphic DNA markers. We have obtained up to 90% accuracy for the classification of alleles in good quality images and up to 70% accuracy in average quality images. These results are obtained within a few seconds. Even after subsequent interactive checking to increase the accuracy of allele classification to 100%, the overall speed with which the data can be processed is greatly increased, compared to manual allele classification. |
| first_indexed | 2024-03-07T02:55:22Z |
| format | Conference item |
| id | oxford-uuid:af16bb33-f14b-46cd-83c2-70ca7aaecf88 |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:55:22Z |
| publishDate | 2001 |
| record_format | dspace |
| spelling | oxford-uuid:af16bb33-f14b-46cd-83c2-70ca7aaecf882022-03-27T03:47:18ZAutomatic analysis of agarose gel imagesConference itemhttp://purl.org/coar/resource_type/c_5794uuid:af16bb33-f14b-46cd-83c2-70ca7aaecf88Symplectic Elements at Oxford2001Adiga, PBhomra, ATurri, MGNicod, ADatta, SRJeavons, PMott, RFlint, JMotivation: Automatic tools to speed up routine biological processes are very much sought after in bio-medical research. Much repetitive work in molecular biology, such as allele calling in genetic analysis, can be made semi-automatic or task specific automatic by using existing techniques from computer science and signal processing. Computerized analysis is reproducible and avoids various forms of human error. Semi-automatic techniques with an interactive check on the results speed up the analysis and reduce the error. Results: We have successfully implemented an image processing software package to automatically analyze agarose gel images of polymorphic DNA markers. We have obtained up to 90% accuracy for the classification of alleles in good quality images and up to 70% accuracy in average quality images. These results are obtained within a few seconds. Even after subsequent interactive checking to increase the accuracy of allele classification to 100%, the overall speed with which the data can be processed is greatly increased, compared to manual allele classification. |
| spellingShingle | Adiga, P Bhomra, A Turri, MG Nicod, A Datta, SR Jeavons, P Mott, R Flint, J Automatic analysis of agarose gel images |
| title | Automatic analysis of agarose gel images |
| title_full | Automatic analysis of agarose gel images |
| title_fullStr | Automatic analysis of agarose gel images |
| title_full_unstemmed | Automatic analysis of agarose gel images |
| title_short | Automatic analysis of agarose gel images |
| title_sort | automatic analysis of agarose gel images |
| work_keys_str_mv | AT adigap automaticanalysisofagarosegelimages AT bhomraa automaticanalysisofagarosegelimages AT turrimg automaticanalysisofagarosegelimages AT nicoda automaticanalysisofagarosegelimages AT dattasr automaticanalysisofagarosegelimages AT jeavonsp automaticanalysisofagarosegelimages AT mottr automaticanalysisofagarosegelimages AT flintj automaticanalysisofagarosegelimages |