In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line.
A continuous cell line, Aa23, was established from eggs of a strain of the Asian tiger mosquito, Aedes albopictus, naturally infected with the intracellular symbiont Wolbachia pipientis. The resulting cell line was shown to be persistently infected with the bacterial endosymbiont. Treatment with ant...
| Asıl Yazarlar: | , , , , , |
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| Materyal Türü: | Journal article |
| Dil: | English |
| Baskı/Yayın Bilgisi: |
1997
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| _version_ | 1826291171476897792 |
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| author | O'Neill, S Pettigrew, M Sinkins, S Braig, H Andreadis, T Tesh, R |
| author_facet | O'Neill, S Pettigrew, M Sinkins, S Braig, H Andreadis, T Tesh, R |
| author_sort | O'Neill, S |
| collection | OXFORD |
| description | A continuous cell line, Aa23, was established from eggs of a strain of the Asian tiger mosquito, Aedes albopictus, naturally infected with the intracellular symbiont Wolbachia pipientis. The resulting cell line was shown to be persistently infected with the bacterial endosymbiont. Treatment with antibiotics cured the cells of the infection. In the course of establishing this cell line it was noticed that RFLPs in the PCR products of two Wolbachia genes from the parental mosquitoes were fixed in the infected cell line. This indicates that the mosquito host was naturally superinfected with different Wolbachia strains, whereas the infected cell line derived from these mosquitoes only contained one of the original Wolbachia strains. The development of an in vitro culture system for this fastidious microorganism should facilitate molecular analysis of the reproduction distorting phenotypes it induces in natural arthropod hosts. |
| first_indexed | 2024-03-07T02:55:24Z |
| format | Journal article |
| id | oxford-uuid:af1a02b8-d60e-4fe9-876f-5c877694a7af |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:55:24Z |
| publishDate | 1997 |
| record_format | dspace |
| spelling | oxford-uuid:af1a02b8-d60e-4fe9-876f-5c877694a7af2022-03-27T03:47:26ZIn vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:af1a02b8-d60e-4fe9-876f-5c877694a7afEnglishSymplectic Elements at Oxford1997O'Neill, SPettigrew, MSinkins, SBraig, HAndreadis, TTesh, RA continuous cell line, Aa23, was established from eggs of a strain of the Asian tiger mosquito, Aedes albopictus, naturally infected with the intracellular symbiont Wolbachia pipientis. The resulting cell line was shown to be persistently infected with the bacterial endosymbiont. Treatment with antibiotics cured the cells of the infection. In the course of establishing this cell line it was noticed that RFLPs in the PCR products of two Wolbachia genes from the parental mosquitoes were fixed in the infected cell line. This indicates that the mosquito host was naturally superinfected with different Wolbachia strains, whereas the infected cell line derived from these mosquitoes only contained one of the original Wolbachia strains. The development of an in vitro culture system for this fastidious microorganism should facilitate molecular analysis of the reproduction distorting phenotypes it induces in natural arthropod hosts. |
| spellingShingle | O'Neill, S Pettigrew, M Sinkins, S Braig, H Andreadis, T Tesh, R In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title | In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title_full | In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title_fullStr | In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title_full_unstemmed | In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title_short | In vitro cultivation of Wolbachia pipientis in an Aedes albopictus cell line. |
| title_sort | in vitro cultivation of wolbachia pipientis in an aedes albopictus cell line |
| work_keys_str_mv | AT oneills invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline AT pettigrewm invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline AT sinkinss invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline AT braigh invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline AT andreadist invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline AT teshr invitrocultivationofwolbachiapipientisinanaedesalbopictuscellline |