A high-mass Q-TOF for the analysis of protein complexes
A tandem mass spectrometer (a modified Q-TOF) for the analysis of protein complexes was discussed. With nanoflow-ESI, large biomolecular assemblies were amenable to MS analysis in native environments. Using the instrument, complexes of the bacterial GroEL chaperonin were detected as intact molecular...
| Main Authors: | , , , |
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| Format: | Conference item |
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2002
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| _version_ | 1826291289935577088 |
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| author | Sobott, F McCammon, MG Hernández, H Robinson, C |
| author_facet | Sobott, F McCammon, MG Hernández, H Robinson, C |
| author_sort | Sobott, F |
| collection | OXFORD |
| description | A tandem mass spectrometer (a modified Q-TOF) for the analysis of protein complexes was discussed. With nanoflow-ESI, large biomolecular assemblies were amenable to MS analysis in native environments. Using the instrument, complexes of the bacterial GroEL chaperonin were detected as intact molecular ions around m/z 12,000-14,000. It was found that isolation of single, well-resolved charge states and subsequent collision-induced dissociation (CID) leads to the formation of ions from individual subunits and subcomplexes. |
| first_indexed | 2024-03-07T02:57:12Z |
| format | Conference item |
| id | oxford-uuid:afba2427-594c-45d6-a1e2-46510c63557f |
| institution | University of Oxford |
| last_indexed | 2024-03-07T02:57:12Z |
| publishDate | 2002 |
| record_format | dspace |
| spelling | oxford-uuid:afba2427-594c-45d6-a1e2-46510c63557f2022-03-27T03:51:22ZA high-mass Q-TOF for the analysis of protein complexesConference itemhttp://purl.org/coar/resource_type/c_5794uuid:afba2427-594c-45d6-a1e2-46510c63557fSymplectic Elements at Oxford2002Sobott, FMcCammon, MGHernández, HRobinson, CA tandem mass spectrometer (a modified Q-TOF) for the analysis of protein complexes was discussed. With nanoflow-ESI, large biomolecular assemblies were amenable to MS analysis in native environments. Using the instrument, complexes of the bacterial GroEL chaperonin were detected as intact molecular ions around m/z 12,000-14,000. It was found that isolation of single, well-resolved charge states and subsequent collision-induced dissociation (CID) leads to the formation of ions from individual subunits and subcomplexes. |
| spellingShingle | Sobott, F McCammon, MG Hernández, H Robinson, C A high-mass Q-TOF for the analysis of protein complexes |
| title | A high-mass Q-TOF for the analysis of protein complexes |
| title_full | A high-mass Q-TOF for the analysis of protein complexes |
| title_fullStr | A high-mass Q-TOF for the analysis of protein complexes |
| title_full_unstemmed | A high-mass Q-TOF for the analysis of protein complexes |
| title_short | A high-mass Q-TOF for the analysis of protein complexes |
| title_sort | high mass q tof for the analysis of protein complexes |
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