The engineering and control of cell-toxic nanoparticles
<p>In this project, native and functionalised mesoporous silica nanoparticles (MSNs) are synthesised and explored for their use as antibacterial delivery vehicles. These antibacterial loaded MSNs contribute significantly to the developing field of nanomedicine. This work broadly entails nanopa...
| Main Authors: | , |
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| Format: | Thesis |
| Language: | English |
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2012
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| _version_ | 1826291292343107584 |
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| author | Mehta, R radhika mehta |
| author2 | Davis, J |
| author_facet | Davis, J Mehta, R radhika mehta |
| author_sort | Mehta, R |
| collection | OXFORD |
| description | <p>In this project, native and functionalised mesoporous silica nanoparticles (MSNs) are synthesised and explored for their use as antibacterial delivery vehicles. These antibacterial loaded MSNs contribute significantly to the developing field of nanomedicine. This work broadly entails nanoparticle generation, characterisation, functionalisation, nanoparticle-bacteria cell viability studies and cell confocal imaging. Silica nanoparticles possess tunable surface chemistry, are thermally stable up to 800°C and are suitable for cell-study due to their low toxicity at sub-100 μg/ml concentrations. The preparation of silica nanoparticles with mesopores offers great potential for their use in a variety of applications; by loading or doping them with drugs, dyes, and imaging agents. In the present study, mesoporous silica nanoparticles with amino, carboxyl, PEG and lactose functionalities are prepared and characterised to determine their size, surface charge, surface area and purity using techniques including transmission electron microscope, dynamic light scattering, zeta potential, nitrogen adsorption-desorption and thermo gravimetric analysis. These particles are subsequently loaded with hydrophobic and volatile antibacterial oils- allyl isothiocyanate and cinnamaldehyde to determine the delivery dosage, release kinetics and need for capping. The silica surface is functionalised with lactose as a capping agent due to its inherent biocompatibility and potential for bio-triggering of hydrolysis in bacterial cells such as Escherichia coli. It is hoped that lactose acts as a suitable stopper via cross-linking the surface with intramolecular hydrogen bonding. Cell viability assays are carried out with antibacterial loaded MSNs to demonstrate bacterial cell killing and its killing efficiency. Thus the mesoporous nanoparticles are envisaged as efficient and colloidally stable antibacterial delivery systems.</p> |
| first_indexed | 2024-03-07T02:57:14Z |
| format | Thesis |
| id | oxford-uuid:afbbbedf-9820-478d-8f26-ea6235449a52 |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:57:14Z |
| publishDate | 2012 |
| record_format | dspace |
| spelling | oxford-uuid:afbbbedf-9820-478d-8f26-ea6235449a522022-03-27T03:51:31ZThe engineering and control of cell-toxic nanoparticlesThesishttp://purl.org/coar/resource_type/c_bdccuuid:afbbbedf-9820-478d-8f26-ea6235449a52Inorganic chemistryNanomaterialsEnglish2012Mehta, Rradhika mehtaDavis, J<p>In this project, native and functionalised mesoporous silica nanoparticles (MSNs) are synthesised and explored for their use as antibacterial delivery vehicles. These antibacterial loaded MSNs contribute significantly to the developing field of nanomedicine. This work broadly entails nanoparticle generation, characterisation, functionalisation, nanoparticle-bacteria cell viability studies and cell confocal imaging. Silica nanoparticles possess tunable surface chemistry, are thermally stable up to 800°C and are suitable for cell-study due to their low toxicity at sub-100 μg/ml concentrations. The preparation of silica nanoparticles with mesopores offers great potential for their use in a variety of applications; by loading or doping them with drugs, dyes, and imaging agents. In the present study, mesoporous silica nanoparticles with amino, carboxyl, PEG and lactose functionalities are prepared and characterised to determine their size, surface charge, surface area and purity using techniques including transmission electron microscope, dynamic light scattering, zeta potential, nitrogen adsorption-desorption and thermo gravimetric analysis. These particles are subsequently loaded with hydrophobic and volatile antibacterial oils- allyl isothiocyanate and cinnamaldehyde to determine the delivery dosage, release kinetics and need for capping. The silica surface is functionalised with lactose as a capping agent due to its inherent biocompatibility and potential for bio-triggering of hydrolysis in bacterial cells such as Escherichia coli. It is hoped that lactose acts as a suitable stopper via cross-linking the surface with intramolecular hydrogen bonding. Cell viability assays are carried out with antibacterial loaded MSNs to demonstrate bacterial cell killing and its killing efficiency. Thus the mesoporous nanoparticles are envisaged as efficient and colloidally stable antibacterial delivery systems.</p> |
| spellingShingle | Inorganic chemistry Nanomaterials Mehta, R radhika mehta The engineering and control of cell-toxic nanoparticles |
| title | The engineering and control of cell-toxic nanoparticles |
| title_full | The engineering and control of cell-toxic nanoparticles |
| title_fullStr | The engineering and control of cell-toxic nanoparticles |
| title_full_unstemmed | The engineering and control of cell-toxic nanoparticles |
| title_short | The engineering and control of cell-toxic nanoparticles |
| title_sort | engineering and control of cell toxic nanoparticles |
| topic | Inorganic chemistry Nanomaterials |
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