Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae.
Starting with 7.7 mg of a beta-tubulin isolated from myxamoebae of the slime mould Physarum polycephalum, 90% of the sequence has been determined by the Edman degradation of peptides generated by cyanogen bromide, trypsin and Staphylococcus aureus protease. Differences to other beta-tubulins are mai...
| Main Authors: | , , , , |
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| Format: | Journal article |
| Language: | English |
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1986
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| _version_ | 1797089017026576384 |
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| author | Singhofer-Wowra, M Clayton, L Dawson, P Gull, K Little, M |
| author_facet | Singhofer-Wowra, M Clayton, L Dawson, P Gull, K Little, M |
| author_sort | Singhofer-Wowra, M |
| collection | OXFORD |
| description | Starting with 7.7 mg of a beta-tubulin isolated from myxamoebae of the slime mould Physarum polycephalum, 90% of the sequence has been determined by the Edman degradation of peptides generated by cyanogen bromide, trypsin and Staphylococcus aureus protease. Differences to other beta-tubulins are mainly conservative and spread evenly throughout the chain except for a high concentration at the C-terminus. The Physarum beta-tubulin shows most homology to Chlamydomonas beta-tubulin (90.5%) and least homology to yeast beta-tubulin (S. cerevisiae, 73.4%). Two tryptic peptides were isolated in approximately equal quantities which were identical except in one position (S/ALTVPELTQRMFDA) showing that at least two beta-tubulins are present in myxamoebae. However, since this was the only heterogeneity found, these beta-tubulins are probably very similar. |
| first_indexed | 2024-03-07T02:58:25Z |
| format | Journal article |
| id | oxford-uuid:b01d1d46-c6b8-41aa-bcf8-2ccf78436b2e |
| institution | University of Oxford |
| language | English |
| last_indexed | 2024-03-07T02:58:25Z |
| publishDate | 1986 |
| record_format | dspace |
| spelling | oxford-uuid:b01d1d46-c6b8-41aa-bcf8-2ccf78436b2e2022-03-27T03:54:08ZAmino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae.Journal articlehttp://purl.org/coar/resource_type/c_dcae04bcuuid:b01d1d46-c6b8-41aa-bcf8-2ccf78436b2eEnglishSymplectic Elements at Oxford1986Singhofer-Wowra, MClayton, LDawson, PGull, KLittle, MStarting with 7.7 mg of a beta-tubulin isolated from myxamoebae of the slime mould Physarum polycephalum, 90% of the sequence has been determined by the Edman degradation of peptides generated by cyanogen bromide, trypsin and Staphylococcus aureus protease. Differences to other beta-tubulins are mainly conservative and spread evenly throughout the chain except for a high concentration at the C-terminus. The Physarum beta-tubulin shows most homology to Chlamydomonas beta-tubulin (90.5%) and least homology to yeast beta-tubulin (S. cerevisiae, 73.4%). Two tryptic peptides were isolated in approximately equal quantities which were identical except in one position (S/ALTVPELTQRMFDA) showing that at least two beta-tubulins are present in myxamoebae. However, since this was the only heterogeneity found, these beta-tubulins are probably very similar. |
| spellingShingle | Singhofer-Wowra, M Clayton, L Dawson, P Gull, K Little, M Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title | Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title_full | Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title_fullStr | Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title_full_unstemmed | Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title_short | Amino-acid sequence data of beta-tubulin from Physarum polycephalum myxamoebae. |
| title_sort | amino acid sequence data of beta tubulin from physarum polycephalum myxamoebae |
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